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Science 22 June 1990:
Vol. 248. no. 4962, pp. 1539 - 1541
DOI: 10.1126/science.2163108
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Articles

Science, Vol 248, Issue 4962, 1539-1541
Copyright © 1990 by American Association for the Advancement of Science

articles

Antibodies to clathrin inhibit endocytosis but not recycling to the trans Golgi network in vitro

RK Draper, Y Goda, FM Brodsky, and Pfeffer SR

Department of Biochemistry, Stanford University School of Medicine, CA 94305-5307.

Mannose 6-phosphate receptors carry newly synthesized lysosomal enzymes from the trans Golgi network (TGN) to prelysosomes and then return to the TGN to carry out another round of lysosomal enzyme delivery. Although clathrin-coated vesicles mediate the export of mannose 6-phosphate receptors from the TGN, nothing is known about the transport vesicles used to carry these receptors back to the TGN. Two different in vitro assays used in this study show that an antibody that interferes with clathrin assembly blocks receptor-mediated endocytosis of transferrin, but has no effect on the recycling of the 300-kilodalton mannose 6-phosphate receptor from prelysosomes to the TGN. These results suggest that the transport of mannose 6-phosphate receptors from prelysosomes to the TGN does not involve clathrin.


THIS ARTICLE HAS BEEN CITED BY OTHER ARTICLES:
In Vitro Formation of Recycling Vesicles from Endosomes Requires Adaptor Protein-1/Clathrin and Is Regulated by Rab4 and the Connector Rabaptin-5.
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Mol. Biol. Cell 15, 4990-5000
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Efficient endosome-to-Golgi transport of Shiga toxin is dependent on dynamin and clathrin.
S. U. Lauvrak, M. L. Torgersen, and K. Sandvig (2004)
J. Cell Sci. 117, 2321-2331
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Vps10p Cycles between the TGN and the Late Endosome via the Plasma Membrane in Clathrin Mutants.
O. Deloche and R. W. Schekman (2002)
Mol. Biol. Cell 13, 4296-4307
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The Epithelial Cell Cytoskeleton and Intracellular Trafficking: I. Shiga toxin B-subunit system: retrograde transport, intracellular vectorization, and more.
L. Johannes (2002)
Am J Physiol Gastrointest Liver Physiol 283, G1-G7
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Visualization of Rab9-mediated vesicle transport from endosomes to the trans-Golgi in living cells.
P. Barbero, L. Bittova, and S. R. Pfeffer (2002)
J. Cell Biol. 156, 511-518
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Role for Dynamin in Late Endosome Dynamics and Trafficking of the Cation-independent Mannose 6-Phosphate Receptor.
P. Nicoziani, F. Vilhardt, A. Llorente, L. Hilout, P. J. Courtoy, K. Sandvig, and B. van Deurs (2000)
Mol. Biol. Cell 11, 481-495
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Vesicle-associated Membrane Protein 4 is Implicated in Trans-Golgi Network Vesicle Trafficking.
M. Steegmaier, J. Klumperman, D. L. Foletti, J.-S. Yoo, and R. H. Scheller (1999)
Mol. Biol. Cell 10, 1957-1972
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Role of Clathrin-coated Vesicles in Glycoprotein Transport from the Cell Surface to the Golgi Complex.
C. R. Bos, S. L. Shank, and M. D. Snider (1995)
J. Biol. Chem. 270, 665-671
   Abstract »    Full Text »    PDF »
Visualization of Rab9-mediated vesicle transport from endosomes to the trans-Golgi in living cells.
P. Barbero, L. Bittova, and S. R. Pfeffer (2002)
J. Cell Biol. 156, 511-518
   Abstract »    Full Text »    PDF »
Dynamin-dependent Transferrin Receptor Recycling by Endosome-derived Clathrin-coated Vesicles.
E. M. van Dam and W. Stoorvogel (2002)
Mol. Biol. Cell 13, 169-182
   Abstract »    Full Text »    PDF »


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