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Science 15 June 1990:
Vol. 248. no. 4961, pp. 1413 - 1416
DOI: 10.1126/science.1972597
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Articles

Science, Vol 248, Issue 4961, 1413-1416
Copyright © 1990 by American Association for the Advancement of Science

articles

Correction of CD18-deficient lymphocytes by retrovirus-mediated gene transfer

JM Wilson, AJ Ping, JC Krauss, L Mayo-Bond, CE Rogers, DC Anderson, and RF Todd

Howard Hughes Medical Institute, Department of Internal Medicine, Ann Arbor, MI.

Leukocyte adhesion deficiency (LAD) is an inherited disorder of leukocyte function caused by derangements in CD18 expression. The genetic and functional abnormalities in a lymphocyte cell line from a patient with LAD have been corrected by retrovirus-mediated transduction of a functional CD18 gene. Lymphocytes from patients with LAD were exposed to CD18-expressing retrovirus and enriched for cells that express CD11a and CD18 (LFA-1) on the cell surface. Molecular and functional analyses of these cells revealed (i) one copy of proviral sequence per cell, (ii) viral-directed CD18 RNA that exceeded normal endogenous levels, (iii) normal quantities of CD11a and CD18 protein on the cell surface, and (iv) reconstitution of LFA-1-dependent adhesive function.


THIS ARTICLE HAS BEEN CITED BY OTHER ARTICLES:
Retroviral-Mediated Gene Transfer of the Leukocyte Integrin CD18 Into Peripheral Blood CD34+ Cells Derived From a Patient With Leukocyte Adhesion Deficiency Type 1 .
T. R. Bauer Jr, B. R. Schwartz, W. Conrad Liles, H. D. Ochs, and D. D. Hickstein (1998)
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Leukocyte Adhesion Deficiency Presenting With Recurrent Otitis Media and Persistent Leukocytosis.
L. M. Voss and K. H. Rhodes (1992)
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In vitro and in vivo consequences of VLA-2 expression on rhabdomyosarcoma cells.
B. Chan, N Matsuura, Y Takada, B. Zetter, and M. Hemler (1991)
Science 251, 1600-1602
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A SUCCESSFUL FIRST STEP IN GENE THERAPY.
(1990)
Journal Watch (General) 1990, 7
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