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Science 25 May 1990:
Vol. 248. no. 4958, pp. 1003 - 1006
DOI: 10.1126/science.2343304
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Articles

Science, Vol 248, Issue 4958, 1003-1006
Copyright © 1990 by American Association for the Advancement of Science

articles

Reversal of creatine kinase translational repression by 3' untranslated sequences

JL Ch'ng, DL Shoemaker, P Schimmel, and EW Holmes

Department of Medicine, Duke University, Durham, NC 27710.

A subline of U937 cells (U937D) was obtained in which creatine kinase B (CK-B) messenger RNA was present and bound to ribosomes, but CK activity was undetectable. Transformation of U937D cells with retrovirus vectors that contain the 3' untranslated region (3' UTR) of CK-B messenger RNA exhibited CK activity with no change in abundance of CK-B mRNA. The 3' UTR formed a complex in vitro with a component of S100 extracts from wild-type cells. This binding activity was not detectable in S100 extracts from cells that expressed CK activity after transformation with the 3' UTR-containing vector. These results suggest that translation of CK-B is repressed by binding of a soluble factor or factors to the 3' UTR.


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