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Science 18 May 1990:
Vol. 248. no. 4957, pp. 854 - 857
DOI: 10.1126/science.2111579
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Articles

Science, Vol 248, Issue 4957, 854-857
Copyright © 1990 by American Association for the Advancement of Science

articles

Relation of neuronal APP-751/APP-695 mRNA ratio and neuritic plaque density in Alzheimer's disease

SA Johnson, T McNeill, B Cordell, and CE Finch

Andrus Gerontology Center, University of Southern California, Los Angeles 90089.

An ongoing controversy concerns the cellular distribution of the differentially spliced forms of the amyloid protein precursor (APP) mRNAs and changes in prevalence of these transcripts during Alzheimer's disease. In situ hybridization on serial sections was used to prove that most hippocampal pyramidal neurons contain both APP-751 and APP-695 mRNA species. The APP-751/APP-695 mRNA ratio is generally increased during Alzheimer's disease, as shown by RNA gel blot analysis. Moreover, there was a strong linear relation between the increase in APP-751/APP-695 mRNA ratio in pyramidal neurons and the density of senile plaques within the hippocampus and entorhinal cortex. Thus, the increase in APP-751/APP-695 mRNA provides a molecular marker for regional variations in plaque density between individuals diagnosed with Alzheimer's disease by the commonly used composite criteria.


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