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Science 11 May 1990:
Vol. 248. no. 4956, pp. 727 - 730
DOI: 10.1126/science.1692159
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Articles

Science, Vol 248, Issue 4956, 727-730
Copyright © 1990 by American Association for the Advancement of Science

articles

Cloning of a 67-kD neutrophil oxidase factor with similarity to a noncatalytic region of p60c-src

TL Leto, KJ Lomax, BD Volpp, H Nunoi, JM Sechler, WM Nauseef, RA Clark, JI Gallin, and HL Malech

Laboratory of Clinical Investigation, National Institute of Allergy and Infectious Diseases, Bethesda, MD 20892.

Chronic granulomatous diseases (CGDs) are characterized by recurrent infections resulting from impaired superoxide production by a phagocytic cell, nicotinamide adenine dinucleotide phosphate (reduced) (NADPH) oxidase. Complementary DNAs were cloned that encode the 67-kilodalton (kD) cytosolic oxidase factor (p67), which is deficient in 5% of CGD patients. Recombinant p67 (r-p67) partially restored NADPH oxidase activity to p67-deficient neutrophil cytosol from these patients. The p67 cDNA encodes a 526-amino acid protein with acidic middle and carboxyl-terminal domains that are similar to a sequence motif found in the noncatalytic domain of src-related tyrosine kinases. This motif was recently noted in phospholipase C-gamma, nonerythroid alpha-spectrin (fodrin), p21ras-guanosine triphophatase-activating protein (GAP), myosin-1 isoforms, yeast proteins cdc-25 and fus-1, and the 47-kD phagocyte oxidase factor (p47), which suggests the possibility of common regulatory features.


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The Rac Target NADPH Oxidase p67[IMAGE] Interacts Preferentially with Rac2 Rather Than Rac1.
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A Domain of p47[IMAGE] That Interacts with Human Neutrophil Flavocytochrome b[IMAGE].
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J. Biol. Chem. 270, 16428-16434
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Repressor Activity of CCAAT Displacement Protein in HL-60 Myeloid Leukemia Cells.
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Characterization of a gp91-phox Promoter Element That Is Required for Interferon [IMAGE]-induced Transcription.
E. A. Eklund and D. G. Skalnik (1995)
J. Biol. Chem. 270, 8267-8273
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Interaction of Rac with p67phox and regulation of phagocytic NADPH oxidase activity.
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Science 265, 531-533
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Binding of the alpha-fodrin SH3 domain to the leading lamellae of locomoting chicken fibroblasts.
J Merilainen, R Palovuori, R Sormunen, V. Wasenius, and V. Lehto (1993)
J. Cell Sci. 105, 647-654
   Abstract »    PDF »
Cytochrome b558: the flavin-binding component of the phagocyte NADPH oxidase.
D Rotrosen, C. Yeung, T. Leto, H. Malech, and C. Kwong (1992)
Science 256, 1459-1462
   Abstract »    PDF »
SH2 and SH3 domains: elements that control interactions of cytoplasmic signaling proteins.
C. Koch, D Anderson, M. Moran, C Ellis, and T Pawson (1991)
Science 252, 668-674
   Abstract »    PDF »
Fused p47phox and p67phox Truncations Efficiently Reconstitute NADPH Oxidase with Higher Activity and Stability Than the Individual Components.
K. Ebisu, T. Nagasawa, K. Watanabe, K. Kakinuma, K. Miyano, and M. Tamura (2001)
J. Biol. Chem. 276, 24498-24505
   Abstract »    Full Text »    PDF »
SHP1 Protein-tyrosine Phosphatase Inhibits gp91PHOX and p67PHOX Expression by Inhibiting Interaction of PU.1, IRF1, Interferon Consensus Sequence-binding Protein, and CREB-binding Protein with Homologous Cis Elements in the CYBB and NCF2 Genes.
B. Kautz, R. Kakar, E. David, and E. A. Eklund (2001)
J. Biol. Chem. 276, 37868-37878
   Abstract »    Full Text »    PDF »
Tyrosine Phosphorylation of HoxA10 Decreases DNA Binding and Transcriptional Repression during Interferon gamma -induced Differentiation of Myeloid Leukemia Cell Lines.
E. A. Eklund, A. Jalava, and R. Kakar (2000)
J. Biol. Chem. 275, 20117-20126
   Abstract »    Full Text »    PDF »


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