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Science 4 May 1990:
Vol. 248. no. 4955, pp. 603 - 605
DOI: 10.1126/science.1692158
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Articles
Science, Vol 248, Issue 4955, 603-605
Copyright © 1990 by American Association for the Advancement of Science
Human cortical neuronal cell line: establishment from a patient with unilateral megalencephaly
GV Ronnett,
LD Hester,
JS Nye,
K Connors,
and
SH Snyder
Department of Neurology, Johns Hopkins University School of Medicine, Baltimore, MD 21205.
A cell line has been established in continuous culture of human cerebral cortical neurons obtained from a patient with unilateral megalencephaly, a disorder associated with continued proliferation of immature neuronal cells. When differentiated in the presence of nerve growth factor, 1-isobutyl-3-methylxanthine, and dibutyryl adenosine 3',5'-monophosphate (cAMP), the cells display mature neuronal morphology with numerous long, extensively branched processes with spines and varicosities. The cells stain positively for neurofilament protein and neuron-specific enolase (selective neuronal markers) but are negative for glial markers, such as glial fibrillary acidic protein, S-100, and myelin basic protein. The cells also stain positively for the neurotransmitters gamma-aminobutyric acid (GABA), glutamate, somatostatin, cholecystokinin-8, and vasoactive intestinal polypeptide. These cells may facilitate characterization of neurons in the human central nervous system.
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