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Science 6 April 1990:
Vol. 248. no. 4951, pp. 64 - 66
DOI: 10.1126/science.2157285
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Articles

Science, Vol 248, Issue 4951, 64-66
Copyright © 1990 by American Association for the Advancement of Science

articles

Molecular cloning and expression of a complementary DNA for inositol 1,4,5-trisphosphate 3-kinase

KY Choi, HK Kim, SY Lee, KH Moon, SS Sim, JW Kim, HK Chung, and SG Rhee

Laboratory of Biochemistry, National Heart, Lung, and Blood Institute, National Institutes of Health, Bethesda, MD 20892.

A complementary DNA (cDNA) clone that encodes inositol 1,4,5-trisphosphate 3-kinase was isolated from a rat brain cDNA expression library with the use of monoclonal antibodies. This clone had an open reading frame that would direct the synthesis of a protein consisting of 449 amino acids and with a molecular mass of 49,853 daltons. The putative protein revealed a potential calmodulin-binding site and six regions with amino acid compositions (PEST regions) common to proteins that are susceptible to calpain. Expression of the cDNA in COS cells resulted in an approximately 150-fold increase in inositol 1,4,5-trisphosphate 3-kinase activity of these cells.


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Isolation of Inositol 1,3,4-Trisphosphate 5/6-Kinase, cDNA Cloning, and Expression of the Recombinant Enzyme.
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