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Science 23 March 1990:
Vol. 247. no. 4949, pp. 1465 - 1468
DOI: 10.1126/science.1690918
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Articles

Science, Vol 247, Issue 4949, 1465-1468
Copyright © 1990 by American Association for the Advancement of Science

articles

Direct gene transfer into mouse muscle in vivo

JA Wolff, RW Malone, P Williams, W Chong, G Acsadi, A Jani, and PL Felgner

Department of Pediatrics, Waisman Center, University of Wisconsin, Madison 53706.

RNA and DNA expression vectors containing genes for chloramphenicol acetyltransferase, luciferase, and beta-galactosidase were separately injected into mouse skeletal muscle in vivo. Protein expression was readily detected in all cases, and no special delivery system was required for these effects. The extent of expression from both the RNA and DNA constructs was comparable to that obtained from fibroblasts transfected in vitro under optimal conditions. In situ cytochemical staining for beta-galactosidase activity was localized to muscle cells following injection of the beta-galactosidase DNA vector. After injection of the DNA luciferase expression vector, luciferase activity was present in the muscle for at least 2 months.


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DNA Vaccines Expressing a Fusion Product of Outer Surface Proteins A and C from Borrelia burgdorferi Induce Protective Antibodies Suitable for Prophylaxis but Not for Resolution of Lyme Disease.
R. Wallich, A. Siebers, O. Jahraus, C. Brenner, T. Stehle, and M. M. Simon (2001)
Infect. Immun. 69, 2130-2136
   Abstract »    Full Text »    PDF »
Induction of a Cellular and Humoral Immune Response against Preprocalcitonin by Genetic Immunization: A Potential New Treatment for Medullary Thyroid Carcinoma.
K. Haupt, F. Siegel, M. Lu, D. Yang, G. Hilken, K. Mann, M. Roggendorf, and B. Saller (2001)
Endocrinology 142, 1017-1023
   Abstract »    Full Text »    PDF »
Vaccination with Allergen-IL-18 Fusion DNA Protects Against, and Reverses Established, Airway Hyperreactivity in a Murine Asthma Model.
H. T. Maecker, G. Hansen, D. M. Walter, R. H. DeKruyff, S. Levy, and D. T. Umetsu (2001)
J. Immunol. 166, 959-965
   Abstract »    Full Text »    PDF »
DNA vaccination: antigen presentation and the induction of immunity.
D. J. Shedlock and D. B. Weiner (2000)
J. Leukoc. Biol. 68, 793-806
   Abstract »    Full Text »
In-vivo transfection of the female reproductive tract epithelium.
M. Relloso and P. Esponda (2000)
Mol. Hum. Reprod. 6, 1099-1105
   Abstract »    Full Text »    PDF »
Control of Viremia and Prevention of Clinical AIDS in Rhesus Monkeys by Cytokine-Augmented DNA Vaccination.
D. H. Barouch, S. Santra, J. E. Schmitz, M. J. Kuroda, T.-M. Fu, W. Wagner, M. Bilska, A. Craiu, X. X. Zheng, G. R. Krivulka, et al. (2000)
Science 290, 486-492
   Abstract »    Full Text »
Animal models for muscular dystrophy: valuable tools for the development of therapies.
V. Allamand and K. P. Campbell (2000)
Hum. Mol. Genet. 9, 2459-2467
   Abstract »    Full Text »    PDF »
Genetic Immunization of BALB/c mice with a Plasmid Bearing the Gene Coding for a Hybrid Merozoite Surface Protein 1-Hepatitis B Virus Surface Protein Fusion Protects Mice against Lethal Plasmodium chabaudi chabaudi PC1 Infection.
G. Wunderlich, I. C. Moura, and H. A. del Portillo (2000)
Infect. Immun. 68, 5839-5845
   Abstract »    Full Text »    PDF »
Antigen Presentation and DNA Vaccines.
J. J. DONNELLY, M. A. LIU, and J. B. ULMER (2000)
Am. J. Respir. Crit. Care Med. 162, S190-193
   Abstract »    Full Text »    PDF »
CD40 Ligand-Dependent Activation of Cytotoxic T Lymphocytes by Adeno-Associated Virus Vectors In Vivo: Role of Immature Dendritic Cells.
Y. Zhang, N. Chirmule, G.-p. Gao, and J. Wilson (2000)
J. Virol. 74, 8003-8010
   Abstract »    Full Text »
Therapeutic Myocardial Angiogenesis Via Vascular Endothelial Growth Factor Gene Therapy : Moving on Down the Road.
C. Patterson and M. S. Runge (2000)
Circulation 102, 940-942
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In Vitro and In Vivo Transfection of p21 Gene Enhances Cyclosporin A-Mediated Inhibition of Lymphocyte Proliferation.
A. K. Khanna and J. D. Hosenpud (2000)
J. Immunol. 165, 1882-1888
   Abstract »    Full Text »    PDF »
DNA Vaccines Encoding Viral Glycoproteins Induce Nonspecific Immunity and Mx Protein Synthesis in Fish.
C. H. Kim, M. C. Johnson, J. D. Drennan, B. E. Simon, E. Thomann, and J.-A. C. Leong (2000)
J. Virol. 74, 7048-7054
   Abstract »    Full Text »


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