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Science 9 March 1990:
Vol. 247. no. 4947, pp. 1213 - 1216
DOI: 10.1126/science.1690452
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Articles

Science, Vol 247, Issue 4947, 1213-1216
Copyright © 1990 by American Association for the Advancement of Science

articles

An intron in the genes for U3 small nucleolar RNAs of the yeast Saccharomyces cerevisiae

E Myslinski, V Segault, and C Branlant

Laboratoire d'Enzymologie et de Genie Genetique, Universite de Nancy, France.

The origin of the intervening sequences (introns), which are removed during RNA maturation, is currently unknown. They are found in most genes encoding messenger RNAs, but are lacking in almost all small nuclear (sn)RNAs. One exceptional snRNA (U6) is part of the spliceosomal machinery that is involved in messenger RNA maturation. It has been suggested that its intron arose as a result of incorrect splicing of a messenger RNA precursor. This study revealed the presence of an intron, with the characteristic features of nuclear introns from precursors to messenger RNA, in the two genes coding for Saccharomyces cerevisiae U3 snRNA. The branch point was GACTAAC instead of the TACTAAC sequence found in all yeast introns examined so far. As U3 is a nucleolar snRNA required for maturation of ribosomal RNA, its intron could not have been acquired from aberrant messenger RNA processing in a spliceosome.


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