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Science 9 February 1990:
Vol. 247. no. 4943, pp. 715 - 718
DOI: 10.1126/science.2137259
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Articles

Science, Vol 247, Issue 4943, 715-718
Copyright © 1990 by American Association for the Advancement of Science

articles

Antigen presentation requires transport of MHC class I molecules from the endoplasmic reticulum

JH Cox, JW Yewdell, LC Eisenlohr, PR Johnson, and Bennink JR

Laboratory of Viral Diseases, National Institute of Allergy and Infectious Diseases, Bethesda, MD 20892.

The role of exocytosis of major histocompatibility complex (MHC) class I molecules in the presentation of antigens to mouse cytotoxic T lymphocytes (CTLs) was examined by use of a recombinant vaccinia virus that expresses the E19 glycoprotein from adenovirus. E19 blocked the presentation of vaccinia and influenza virus proteins to CTLs in a MHC class I allele-specific manner identical to its inhibition of MHC class I transport from the endoplasmic reticulum. This finding indicates that (i) the relevant parameter for antigen presentation is the rate of MHC class I molecule exocytosis, not the level of class I cell surface expression, and (ii) association of class I molecules with antigen is likely to occur within the endoplasmic reticulum.


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