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Science 19 January 1990:
Vol. 247. no. 4940, pp. 320 - 322
DOI: 10.1126/science.2296721
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Articles

Science, Vol 247, Issue 4940, 320-322
Copyright © 1990 by American Association for the Advancement of Science

articles

Identification of geranylgeranyl-modified proteins in HeLa cells

CC Farnsworth, MH Gelb, and JA Glomset

Howard Hughes Medical Institute Laboratory, University of Washington, Seattle 98195.

Previous studies have shown that animal cells contain isoprenoid-modified proteins and that one of these proteins, lamin B, contains a thioether-linked farnesyl group that is attached to cysteine. In the present study, a novel isoprenoid-modification was identified by labeling HeLa cells with [3H]mevalonic acid and analyzing proteolytic digests of the total cell protein. Radioactive fragments were purified from these digests and treated with Raney nickel. The released, labeled material was analyzed by gas-liquid chromatography (GC) and mass spectrometry (MS). This approach revealed that an all-trans geranylgeranyl group was a major isoprenoid modification.


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