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Science 12 January 1990:
Vol. 247. no. 4939, pp. 205 - 209
DOI: 10.1126/science.2104680
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Articles

Science, Vol 247, Issue 4939, 205-209
Copyright © 1990 by American Association for the Advancement of Science

articles

Repression of c-fos transcription and an altered genetic program in senescent human fibroblasts

T Seshadri and J Campisi

Department of Biochemistry, Boston University Medical School, MA 02118.

Normal cells in culture invariably undergo senescence, whereby they cease proliferation after a finite number of doublings. Irreversible changes in gene expression occurred in senescent human fetal lung fibroblasts: a non-cell cycle-regulated mRNA was partially repressed; an unusual polyadenylated histone mRNA was expressed; although serum induced c-H-ras, c-myc, and ornithine decarboxylase mRNA normally, ornithine decarboxylase activity was deficient; and serum did not induce mRNA for a replication-dependent histone and for the c-fos proto-oncogene. The loss of c-fos inducibility was the result of a specific, transcriptional block. The results suggest that senescent fibroblasts were unable to proliferate because of, at least in part, selective repression of c-fos; moreover, the multiple changes in gene expression support the view that cellular senescence is a process of terminal differentiation.


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