Note to users. If you're seeing this message, it means that your browser cannot find this page's style/presentation instructions -- or possibly that you are using a browser that does not support current Web standards. Find out more about why this message is appearing, and what you can do to make your experience of our site the best it can be.

Science 29 September 1989:
Vol. 245. no. 4925, pp. 1499 - 1501
DOI: 10.1126/science.2571189
Prev | Table of Contents | Next

Articles

Science, Vol 245, Issue 4925, 1499-1501
Copyright © 1989 by American Association for the Advancement of Science

articles

Mechanism of membrane anchoring affects polarized expression of two proteins in MDCK cells

DA Brown, B Crise, and JK Rose

Department of Pathology, Yale University School of Medicine, New Haven, CT 06510.

The signals that direct membrane proteins to the apical or basolateral plasma membrane domains of polarized epithelial cells are not known. Several of the class of proteins anchored in the membrane by glycosyl-phosphatidylinositol (GPI) are expressed on the apical surface of such cells. However, it is not known whether the mechanism of membrane anchorage or the polypeptide sequence provides the sorting information. The conversion of the normally basolateral vesicular stomatitis virus glycoprotein (VSV G) to a GPI-anchored protein led to its apical expression. Conversely, replacement of the GPI anchor of placental alkaline phosphatase with the transmembrane and cytoplasmic domains of VSV G shifted its expression from the apical to the basolateral surface. Thus, the mechanism of membrane anchorage can determine the sorting of proteins to the apical or basolateral surface, and the GPI anchor itself may provide an apical transport signal.


THIS ARTICLE HAS BEEN CITED BY OTHER ARTICLES:
Apical trafficking in epithelial cells: signals, clusters and motors.
O. A. Weisz and E. Rodriguez-Boulan (2009)
J. Cell Sci. 122, 4253-4266
   Abstract »    Full Text »    PDF »
Live-Cell Imaging Reveals Periarbuscular Membrane Domains and Organelle Location in Medicago truncatula Roots during Arbuscular Mycorrhizal Symbiosis.
N. Pumplin and M. J. Harrison (2009)
Plant Physiology 151, 809-819
   Abstract »    Full Text »    PDF »
Selective Roles for Cholesterol and Actin in Compartmentalization of Different Proteins in the Golgi and Plasma Membrane of Polarized Cells.
S. Lebreton, S. Paladino, and C. Zurzolo (2008)
J. Biol. Chem. 283, 29545-29553
   Abstract »    Full Text »    PDF »
Bryostatin 1 modulates {beta}-catenin subcellular localization and transcription activity through protein kinase D1 activation.
M. Jaggi, S. C. Chauhan, C. Du, and K.C. Balaji (2008)
Mol. Cancer Ther. 7, 2703-2712
   Abstract »    Full Text »    PDF »
Hantavirus Causing Hemorrhagic Fever with Renal Syndrome Enters from the Apical Surface and Requires Decay-Accelerating Factor (DAF/CD55).
E. Krautkramer and M. Zeier (2008)
J. Virol. 82, 4257-4264
   Abstract »    Full Text »    PDF »
Distinct v-SNAREs regulate direct and indirect apical delivery in polarized epithelial cells.
T. Pocard, A. Le Bivic, T. Galli, and C. Zurzolo (2007)
J. Cell Sci. 120, 3309-3320
   Abstract »    Full Text »    PDF »
A Bipartite Signal Regulates the Faithful Delivery of Apical Domain Marker Podocalyxin/Gp135.
C.-Y. Yu, J.-Y. Chen, Y.-Y. Lin, K.-F. Shen, W.-L. Lin, C.-L. Chien, M. B.A. ter Beest, and T.-S. Jou (2007)
Mol. Biol. Cell 18, 1710-1722
   Abstract »    Full Text »    PDF »
De Novo Sphingolipid Synthesis Is Essential for Viability, but Not for Transport of Glycosylphosphatidylinositol-Anchored Proteins, in African Trypanosomes.
S. S. Sutterwala, C. H. Creswell, S. Sanyal, A. K. Menon, and J. D. Bangs (2007)
Eukaryot. Cell 6, 454-464
   Abstract »    Full Text »    PDF »
AP1B sorts basolateral proteins in recycling and biosynthetic routes of MDCK cells.
D. Gravotta, A. Deora, E. Perret, C. Oyanadel, A. Soza, R. Schreiner, A. Gonzalez, and E. Rodriguez-Boulan (2007)
PNAS 104, 1564-1569
   Abstract »    Full Text »    PDF »
Cellular uptake of fatty acids driven by the ER-localized acyl-CoA synthetase FATP4.
K. Milger, T. Herrmann, C. Becker, D. Gotthardt, J. Zickwolf, R. Ehehalt, P. A. Watkins, W. Stremmel, and J. Fullekrug (2006)
J. Cell Sci. 119, 4678-4688
   Abstract »    Full Text »    PDF »
Erlin-1 and erlin-2 are novel members of the prohibitin family of proteins that define lipid-raft-like domains of the ER.
D. T. Browman, M. E. Resek, L. D. Zajchowski, and S. M. Robbins (2006)
J. Cell Sci. 119, 3149-3160
   Abstract »    Full Text »    PDF »
Controversy fuels trafficking of GPI-anchored proteins..
S. Schuck and K. Simons (2006)
J. Cell Biol. 172, 963-965
   Abstract »    Full Text »    PDF »
Phase coexistence and connectivity in the apical membrane of polarized epithelial cells.
D. Meder, M. J. Moreno, P. Verkade, W. L. C. Vaz, and K. Simons (2006)
PNAS 103, 329-334
   Abstract »    Full Text »    PDF »
Role of N- and O-glycans in polarized biosynthetic sorting.
B. A. Potter, R. P. Hughey, and O. A. Weisz (2006)
Am J Physiol Cell Physiol 290, C1-C10
   Abstract »    Full Text »    PDF »
Deglycosylation of Na+/K+-ATPase causes the basolateral protein to undergo apical targeting in polarized hepatic cells.
W.-N. Lian, T.-W. Wu, R.-L. Dao, Y.-J. Chen, and C.-H. Lin (2006)
J. Cell Sci. 119, 11-22
   Abstract »    Full Text »    PDF »
Caveolin-1 Is Not Essential for Biosynthetic Apical Membrane Transport.
A. Manninen, P. Verkade, S. Le Lay, J. Torkko, M. Kasper, J. Fullekrug, and K. Simons (2005)
Mol. Cell. Biol. 25, 10087-10096
   Abstract »    Full Text »    PDF »
Parotid Secretory Granules: Crossroads of Secretory Pathways and Protein Storage.
S.-U. Gorr, S.G. Venkatesh, and D.S. Darling (2005)
Journal of Dental Research 84, 500-509
   Abstract »    Full Text »    PDF »
GPI7 Involved in Glycosylphosphatidylinositol Biosynthesis Is Essential for Yeast Cell Separation.
M. Fujita, T. Yoko-o, M. Okamoto, and Y. Jigami (2004)
J. Biol. Chem. 279, 51869-51879
   Abstract »    Full Text »    PDF »
Polarized sorting in epithelial cells: raft clustering and the biogenesis of the apical membrane.
S. Schuck and K. Simons (2004)
J. Cell Sci. 117, 5955-5964
   Abstract »    Full Text »    PDF »
Protein oligomerization modulates raft partitioning and apical sorting of GPI-anchored proteins.
S. Paladino, D. Sarnataro, R. Pillich, S. Tivodar, L. Nitsch, and C. Zurzolo (2004)
J. Cell Biol. 167, 699-709
   Abstract »    Full Text »    PDF »
N-Glycans, not the GPI anchor, mediate the apical targeting of a naturally glycosylated, GPI-anchored protein in polarised epithelial cells.
S. Pang, P. Urquhart, and N. M. Hooper (2004)
J. Cell Sci. 117, 5079-5086
   Abstract »    Full Text »    PDF »
PrPC Association with Lipid Rafts in the Early Secretory Pathway Stabilizes Its Cellular Conformation.
D. Sarnataro, V. Campana, S. Paladino, M. Stornaiuolo, L. Nitsch, and C. Zurzolo (2004)
Mol. Biol. Cell 15, 4031-4042
   Abstract »    Full Text »    PDF »
The COOH-terminal tail of the GAT-2 GABA transporter contains a novel motif that plays a role in basolateral targeting.
A. Brown, T. Muth, and M. Caplan (2004)
Am J Physiol Cell Physiol 286, C1071-C1077
   Abstract »    Full Text »    PDF »
Bidirectional Transepithelial IgG Transport by a Strongly Polarized Basolateral Membrane Fc{gamma}-Receptor.
S. M. Claypool, B. L. Dickinson, J. S. Wagner, F.-E. Johansen, N. Venu, J. A. Borawski, W. I. Lencer, and R. S. Blumberg (2004)
Mol. Biol. Cell 15, 1746-1759
   Abstract »    Full Text »    PDF »
Association of rat8 with Fyn protein kinase via lipid rafts is required for rat mammary cell differentiation in vitro.
I. Zucchi, A. Prinetti, M. Scotti, V. Valsecchi, R. Valaperta, E. Mento, R. Reinbold, P. Vezzoni, S. Sonnino, A. Albertini, et al. (2004)
PNAS 101, 1880-1885
   Abstract »    Full Text »    PDF »
Transcytosis: Crossing Cellular Barriers.
P. L. TUMA and A. L. HUBBARD (2003)
Physiol Rev 83, 871-932
   Abstract »    Full Text »    PDF »
Sensitivity of Polarized Epithelial Cells to the Pore-Forming Toxin Aerolysin.
L. Abrami, M. Fivaz, P.-E. Glauser, N. Sugimoto, C. Zurzolo, and F. G. van der Goot (2003)
Infect. Immun. 71, 739-746
   Abstract »    Full Text »    PDF »
Raft-mediated Trafficking of Apical Resident Proteins Occurs in Both Direct and Transcytotic Pathways in Polarized Hepatic Cells: Role of Distinct Lipid Microdomains.
T. A. Slimane, G. Trugnan, S. C.D. van IJzendoorn, and D. Hoekstra (2003)
Mol. Biol. Cell 14, 611-624
   Abstract »    Full Text »    PDF »
Essential Role of the Prion Protein N Terminus in Subcellular Trafficking and Half-life of Cellular Prion Protein.
M. Nunziante, S. Gilch, and H. M. Schatzl (2003)
J. Biol. Chem. 278, 3726-3734
   Abstract »    Full Text »    PDF »
The Epidermal Growth Factor Receptor Juxtamembrane Domain Has Multiple Basolateral Plasma Membrane Localization Determinants, Including a Dominant Signal with a Polyproline Core.
C. He, M. Hobert, L. Friend, and C. Carlin (2002)
J. Biol. Chem. 277, 38284-38293
   Abstract »    Full Text »    PDF »
Interaction with Decay-Accelerating Factor Facilitates Coxsackievirus B Infection of Polarized Epithelial Cells.
J. T. C. Shieh and J. M. Bergelson (2002)
J. Virol. 76, 9474-9480
   Abstract »    Full Text »    PDF »
Intracellular Retention of Glycosylphosphatidyl Inositol-Linked Proteins in Caveolin-Deficient Cells.
F. Sotgia, B. Razani, G. Bonuccelli, W. Schubert, M. Battista, H. Lee, F. Capozza, A. L. Schubert, C. Minetti, J. T. Buckley, et al. (2002)
Mol. Cell. Biol. 22, 3905-3926
   Abstract »    Full Text »    PDF »
The Ubiquitin-Proteasome Pathway Regulates the Availability of the GH Receptor.
P. van Kerkhof, M. Smeets, and G. J. Strous (2002)
Endocrinology 143, 1243-1252
   Abstract »    Full Text »    PDF »
Intestinal Dipeptidyl Peptidase IV Is Efficiently Sorted to the Apical Membrane through the Concerted Action of N- and O-Glycans as Well as Association with Lipid Microdomains.
M. Alfalah, R. Jacob, and H. Y. Naim (2002)
J. Biol. Chem. 277, 10683-10690
   Abstract »    Full Text »    PDF »
Mucin-like Domain of Enteropeptidase Directs Apical Targeting in Madin-Darby Canine Kidney Cells.
X. Zheng and J. E. Sadler (2002)
J. Biol. Chem. 277, 6858-6863
   Abstract »    Full Text »    PDF »
The Organizing Potential of Sphingolipids in Intracellular Membrane Transport.
J. C. M. Holthuis, T. Pomorski, R. J. Raggers, H. Sprong, and G. Van Meer (2001)
Physiol Rev 81, 1689-1723
   Abstract »    Full Text »    PDF »
Lipid Rafts and Pseudotyping.
W. F. Pickl, F. X. Pimentel-Muinos, and B. Seed (2001)
J. Virol. 75, 7175-7183
   Abstract »    Full Text »
Identification of an Outer Segment Targeting Signal in the Cooh Terminus of Rhodopsin Using Transgenic Xenopus laevis.
B. M. Tam, O. L. Moritz, L. B. Hurd, and D. S. Papermaster (2000)
J. Cell Biol. 151, 1369-1380
   Abstract »    Full Text »    PDF »
GPI-Anchored Proteins and Glycosphingolipid-Rich Rafts: Platforms for Adhesion and Signaling.
J. Trotter, C. Klein, and E.-M. Kramer (2000)
Neuroscientist 6, 271-284
   Abstract »    PDF »
Retargeting the Coxsackievirus and Adenovirus Receptor to the Apical Surface of Polarized Epithelial Cells Reveals the Glycocalyx as a Barrier to Adenovirus-Mediated Gene Transfer.
R. J. Pickles, J. A. Fahrner, J. M. Petrella, R. C. Boucher, and J. M. Bergelson (2000)
J. Virol. 74, 6050-6057
   Abstract »    Full Text »
The MAL Proteolipid Is Necessary for the Overall Apical Delivery of Membrane Proteins in the Polarized Epithelial Madin-Darby Canine Kidney and Fischer Rat Thyroid Cell Lines.
F. Martín-Belmonte, R. Puertollano, J. Millán, and M. A. Alonso (2000)
Mol. Biol. Cell 11, 2033-2045
   Abstract »    Full Text »
Influenza Virus Assembly and Lipid Raft Microdomains: a Role for the Cytoplasmic Tails of the Spike Glycoproteins.
J. Zhang, A. Pekosz, and R. A. Lamb (2000)
J. Virol. 74, 4634-4644
   Abstract »    Full Text »
Structural Determinants Required for Apical Sorting of an Intestinal Brush-border Membrane Protein.
R. Jacob, M. Alfalah, J. Grunberg, M. Obendorf, and H. Y. Naim (2000)
J. Biol. Chem. 275, 6566-6572
   Abstract »    Full Text »    PDF »
A Transmembrane Segment Determines the Steady-State Localization of an Ion-Transporting Adenosine Triphosphatase.
L. A. Dunbar, P. Aronson, and M. J. Caplan (2000)
J. Cell Biol. 148, 769-778
   Abstract »    Full Text »    PDF »
Induction of Caveolae in the Apical Plasma Membrane of Madin-Darby Canine Kidney Cells.
P. Verkade, T. Harder, F. Lafont, and K. Simons (2000)
J. Cell Biol. 148, 727-740
   Abstract »    Full Text »    PDF »
Detergent-insoluble GPI-anchored Proteins Are Apically Sorted in Fischer Rat Thyroid Cells, but Interference with Cholesterol or Sphingolipids Differentially Affects Detergent Insolubility and Apical Sorting.
C. Lipardi, L. Nitsch, and C. Zurzolo (2000)
Mol. Biol. Cell 11, 531-542
   Abstract »    Full Text »
Intracellular traffic of the ecto-nucleotide pyrophosphatase/phosphodiesterase NPP3 to the apical plasma membrane of MDCK and Caco-2 cells: apical targeting occurs in the absence of N-glycosylation.
N. Meerson, V Bello, J. Delaunay, T. Slimane, D Delautier, C Lenoir, G Trugnan, and M Maurice (2000)
J. Cell Sci. 113, 4193-4202
   Abstract »    PDF »
Carboxypeptidase M, a Glycosylphosphatidylinositol-anchored Protein, Is Localized on Both the Apical and Basolateral Domains of Polarized Madin-Darby Canine Kidney Cells.
G. B. McGwire, R. P. Becker, and R. A. Skidgel (1999)
J. Biol. Chem. 274, 31632-31640
   Abstract »    Full Text »    PDF »
MAL, an Integral Element of the Apical Sorting Machinery, Is an Itinerant Protein That Cycles between the Trans-Golgi Network and the Plasma Membrane.
R. Puertollano and M. A. Alonso (1999)
Mol. Biol. Cell 10, 3435-3447
   Abstract »    Full Text »
N-Glycans Mediate the Apical Sorting of a Gpi-Anchored, Raft-Associated Protein in Madin-Darby Canine Kidney Cells.
J. H. Benting, A. G. Rietveld, and K. Simons (1999)
J. Cell Biol. 146, 313-320
   Abstract »    Full Text »    PDF »
Hierarchy of Sorting Signals in Chimeras of Intestinal Lactase-Phlorizin Hydrolase and the Influenza Virus Hemagglutinin.
R. Jacob, U. Preuss, P. Panzer, M. Alfalah, S. Quack, M. G. Roth, H. Naim, and H. Y. Naim (1999)
J. Biol. Chem. 274, 8061-8067
   Abstract »    Full Text »    PDF »
Resistance of Paroxysmal Nocturnal Hemoglobinuria Cells to the Glycosylphosphatidylinositol-Binding Toxin Aerolysin.
R. A. Brodsky, G. L. Mukhina, K. L. Nelson, T. S. Lawrence, R. J. Jones, and J. T. Buckley (1999)
Blood 93, 1749-1756
   Abstract »    Full Text »    PDF »
Coupling Assembly of the E-Cadherin/{beta}-Catenin Complex to Efficient Endoplasmic Reticulum Exit and Basal-lateral Membrane Targeting of E-Cadherin in Polarized MDCK Cells.
Y.-T. Chen, D. B. Stewart, and W. J. Nelson (1999)
J. Cell Biol. 144, 687-699
   Abstract »    Full Text »    PDF »
Role of Lipid Modifications in Targeting Proteins to Detergent-resistant Membrane Rafts. MANY RAFT PROTEINS ARE ACYLATED, WHILE FEW ARE PRENYLATED.
K. A. Melkonian, A. G. Ostermeyer, J. Z. Chen, M. G. Roth, and D. A. Brown (1999)
J. Biol. Chem. 274, 3910-3917
   Abstract »    Full Text »    PDF »
Endocytosis at the apical plasma membrane of pancreatic acinar cells is regulated by tyrosine kinases.
S. D. Freedman, M. H. Katz, E. M. Parker, and A. Gelrud (1999)
Am J Physiol Cell Physiol 276, C306-C311
   Abstract »    Full Text »    PDF »
Interactions of the AP-1 Golgi Adaptor with the Polymeric Immunoglobulin Receptor and Their Possible Role in Mediating Brefeldin A-sensitive Basolateral Targeting from the trans-Golgi Network.
E. Orzech, K. Schlessinger, A. Weiss, C. T. Okamoto, and B. Aroeti (1999)
J. Biol. Chem. 274, 2201-2215
   Abstract »    Full Text »    PDF »
Apical Sorting of Bovine Enteropeptidase Does Not Involve Detergent-resistant Association with Sphingolipid-Cholesterol Rafts.
X. Zheng, D. Lu, and J. E. Sadler (1999)
J. Biol. Chem. 274, 1596-1605
   Abstract »    Full Text »    PDF »
Polarized targeting of epithelial cell proteins in thyrocytes and MDCK cells.
D Prabakaran, R. Ahima, J. Harney, M. Berry, P. Larsen, and P Arvan (1999)
J. Cell Sci. 112, 1247-1256
   Abstract »    PDF »
New Perspectives on Mechanisms Involved in Generating Epithelial Cell Polarity.
C. YEAMAN, K. K. GRINDSTAFF, and W. J. NELSON (1999)
Physiol Rev 79, 73-98
   Abstract »    Full Text »    PDF »
Putative O-Glycosylation Sites and a Membrane Anchor Are Necessary for Apical Delivery of the Human Neurotrophin Receptor in Caco-2 Cells.
L. Monlauzeur, L. Breuza, and A. Le Bivic (1998)
J. Biol. Chem. 273, 30263-30270
   Abstract »    Full Text »    PDF »
The Cytoplasmic Domains of a beta 1 Integrin Mediate Polarization in Madin-Darby Canine Kidney Cells by Selective Basolateral Stabilization.
A. Gut, M. S. Balda, and K. Matter (1998)
J. Biol. Chem. 273, 29381-29388
   Abstract »    Full Text »    PDF »
Association of GAP-43 with Detergent-resistant Membranes Requires Two Palmitoylated Cysteine Residues.
S. Arni, S. A. Keilbaugh, A. G. Ostermeyer, and D. A. Brown (1998)
J. Biol. Chem. 273, 28478-28485
   Abstract »    Full Text »    PDF »
Tyrosine-based Membrane Protein Sorting Signals Are Differentially Interpreted by Polarized Madin-Darby Canine Kidney and LLC-PK1 Epithelial Cells.
D. L. Roush, C. J. Gottardi, H. Y. Naim, M. G. Roth, and M. J. Caplan (1998)
J. Biol. Chem. 273, 26862-26869
   Abstract »    Full Text »    PDF »
The Cytoplasmic Tail of Rhodopsin Acts as a Novel Apical Sorting Signal in Polarized MDCK Cells.
J.-Z. Chuang and C.-H. Sung (1998)
J. Cell Biol. 142, 1245-1256
   Abstract »    Full Text »    PDF »
Distribution of a Glycosylphosphatidylinositol-anchored Protein at the Apical Surface of MDCK Cells Examined at a Resolution of <100 A Using Imaging Fluorescence Resonance Energy Transfer.
A.K. Kenworthy and M. Edidin (1998)
J. Cell Biol. 142, 69-84
   Abstract »    Full Text »    PDF »
Mutations in the Middle of the Transmembrane Domain Reverse the Polarity of Transport of the Influenza Virus Hemagglutinin in MDCK Epithelial Cells.
S. Lin, H. Y. Naim, A. Chapin Rodriguez, and M. G. Roth (1998)
J. Cell Biol. 142, 51-57
   Abstract »    Full Text »    PDF »
The Protozoan Parasite Toxoplasma gondii Targets Proteins to Dense Granules and the Vacuolar Space Using Both Conserved and Unusual Mechanisms.
V. Karsten, H. Qi, C. J.M. Beckers, A. Reddy, J.-F. Dubremetz, P. Webster, and K. A. Joiner (1998)
J. Cell Biol. 141, 1323-1333
   Abstract »    Full Text »    PDF »
Patterns for RANTES Secretion and Intercellular Adhesion Molecule 1 Expression Mediate Transepithelial T Cell Traffic Based on Analyses In Vitro and In Vivo.
M. Taguchi, D. Sampath, T. Koga, M. Castro, D. C. Look, S. Nakajima, and M. J. Holtzman (1998)
J. Exp. Med. 187, 1927-1940
   Abstract »    Full Text »    PDF »
A Short Peptide Motif at the Carboxyl Terminus Is Required for Incorporation of the Integral Membrane MAL Protein to Glycolipid-enriched Membranes.
R. Puertollano and M. A. Alonso (1998)
J. Biol. Chem. 273, 12740-12745
   Abstract »    Full Text »    PDF »
Lipid Domain Structure of the Plasma Membrane Revealed by Patching of Membrane Components.
T. Harder, P. Scheiffele, P. Verkade, and K. Simons (1998)
J. Cell Biol. 141, 929-942
   Abstract »    Full Text »    PDF »
Cholesterol Is Required for Surface Transport of Influenza Virus Hemagglutinin.
P. Keller and K. Simons (1998)
J. Cell Biol. 140, 1357-1367
   Abstract »    Full Text »    PDF »
Caveolin Transfection Results in Caveolae Formation but Not Apical Sorting of Glycosylphosphatidylinositol (GPI)-anchored Proteins in Epithelial Cells.
C. Lipardi, R. Mora, V. Colomer, S. Paladino, L. Nitsch, E. Rodriguez-Boulan, and C. Zurzolo (1998)
J. Cell Biol. 140, 617-626
   Abstract »    Full Text »    PDF »
A Pore-forming Toxin Interacts with a GPI-anchored Protein and Causes Vacuolation of the Endoplasmic Reticulum.
L. Abrami, M. Fivaz, P.-E. Glauser, R. G. Parton, and F. van der Goot (1998)
J. Cell Biol. 140, 525-540
   Abstract »    Full Text »    PDF »
Basolateral Sorting of the Cation-dependent Mannose 6-Phosphate Receptor in Madin-Darby Canine Kidney Cells. IDENTIFICATION OF A BASOLATERAL DETERMINANT UNRELATED TO CLATHRIN-COATED PIT LOCALIZATION SIGNALS.
B. Distel, U. Bauer, R. Le Borgne, and B. Hoflack (1998)
J. Biol. Chem. 273, 186-193
   Abstract »    Full Text »    PDF »
Biogenesis of tight junctions: the C-terminal domain of occludin mediates basolateral targeting.
K Matter and M. Balda (1998)
J. Cell Sci. 111, 511-519
   Abstract »    PDF »
Multiple Sorting Signals Determine Apical Localization of a Nonglycosylated Integral Membrane Protein.
M. A. Alonso, L. Fan, and B. Alarcon (1997)
J. Biol. Chem. 272, 30748-30752
   Abstract »    Full Text »    PDF »
The O-glycosylated Stalk Domain Is Required for Apical Sorting of Neurotrophin Receptors in Polarized MDCK Cells.
C. Yeaman, A. H. L. Gall, A. N. Baldwin, L. Monlauzeur, A. L. Bivic, and E. Rodriguez-Boulan (1997)
J. Cell Biol. 139, 929-940
   Abstract »    Full Text »    PDF »
Retrovirus-mediated Gene Transfer into Rat Salivary Gland Cells In Vitro and In Vivo.
T. Barka and H. M. van der Noen (1997)
J. Histochem. Cytochem. 45, 1533-1546
   Abstract »    Full Text »    PDF »
Polarized Apical Targeting Directed by the Signal/Anchor Region of Simian Virus 5 Hemagglutinin-Neuraminidase.
X. F. Huang, R. W. Compans, S. Chen, R. A. Lamb, and P. Arvan (1997)
J. Biol. Chem. 272, 27598-27604
   Abstract »    Full Text »    PDF »
Membrane Cofactor Protein (CD46) Is a Basolateral Protein That Is Not Endocytosed. IMPORTANCE OF THE TETRAPEPTIDE FTSL AT THE CARBOXYL TERMINUS.
A. Maisner, G. Zimmer, M. K. Liszewski, D. M. Lublin, J. P. Atkinson, and G. Herrler (1997)
J. Biol. Chem. 272, 20793-20799
   Abstract »    Full Text »    PDF »
Structural Requirements for Basolateral Sorting of the Human Transferrin Receptor in the Biosynthetic and Endocytic Pathways of Madin-Darby Canine Kidney Cells.
G. Odorizzi and I. S. Trowbridge (1997)
J. Cell Biol. 137, 1255-1264
   Abstract »    Full Text »    PDF »
Sorting of Two Polytopic Proteins, the gamma -Aminobutyric Acid and Betaine Transporters, in Polarized Epithelial Cells.
C. Perego, A. Bulbarelli, R. Longhi, M. Caimi, A. Villa, M. J. Caplan, and G. Pietrini (1997)
J. Biol. Chem. 272, 6584-6592
   Abstract »    Full Text »    PDF »
Apical sorting of hepatitis B surface antigen (HBsAg) is independent of N-glycosylation and glycosylphosphatidylinositol-anchored protein segregation.
M. P. Marzolo, P. Bull, and A. Gonzalez (1997)
PNAS 94, 1834-1839
   Abstract »    Full Text »    PDF »
Post-Golgi biosynthetic trafficking.
P Keller and K Simons (1997)
J. Cell Sci. 110, 3001-3009
   Abstract »    PDF »
A Di-hydrophobic Leu-Val Motif Regulates the Basolateral Localization of CD44 in Polarized Madin-Darby Canine Kidney Epithelial Cells.
H. Sheikh and C. M. Isacke (1996)
J. Biol. Chem. 271, 12185-12190
   Abstract »    Full Text »    PDF »
Axonal Amyloid Precursor Protein Expressed by Neurons in Vitro Is Present in a Membrane Fraction with Caveolae-like Properties.
C. Bouillot, A. Prochiantz, G.èv. Rougon, and B. Allinquant (1996)
J. Biol. Chem. 271, 7640-7644
   Abstract »    Full Text »    PDF »
Nonpolarized Distribution of Glycosylphosphatidylinositols in the Plasma Membrane of Polarized Madin-Darby Canine Kidney Cells.
W. v.'t Hof, E. Rodriguez-Boulan, and A. K. Menon (1995)
J. Biol. Chem. 270, 24150-24155
   Abstract »    Full Text »    PDF »
Sorting and Intracellular Trafficking of a Glycosylphosphatidylinositol-anchored Protein and Two Hybrid Transmembrane Proteins with the Same Ectodomain in Madin-Darby Canine Kidney Epithelial Cells.
G. Arreaza and D. A. Brown (1995)
J. Biol. Chem. 270, 23641-23647
   Abstract »    Full Text »    PDF »
Separation of caveolae from associated microdomains of GPI-anchored proteins.
J. Schnitzer, D. McIntosh, A. Dvorak, J Liu, and P Oh (1995)
Science 269, 1435-1439
   Abstract »    PDF »
A Cytoplasmic Tyrosine Is Essential for the Basolateral Localization of Mutants of the Human Nerve Growth Factor Receptor in Madin- Darby Canine Kidney Cells.
L. Monlauzeur, A. Rajasekaran, M. Chao, E. Rodriguez-Boulan, and A. L. Bivic (1995)
J. Biol. Chem. 270, 12219-12225
   Abstract »    Full Text »    PDF »
Epithelial sorting of a glycosylphosphatidylinositol-anchored bacterial protein expressed in polarized renal MDCK and intestinal Caco-2 cells.
K. Soole, M. Jepson, G. Hazlewood, H. Gilbert, and B. Hirst (1995)
J. Cell Sci. 108, 369-377
   Abstract »    PDF »
GPI membrane anchor is determinant in intracellular accumulation of apical plasma membrane proteins in the non-polarized human colon cancer cell line HT-29 18.
F Nicolas, M. Tiveron, J Davoust, and H Reggio (1994)
J. Cell Sci. 107, 2679-2689
   Abstract »    PDF »
Apical plasma membrane proteins are not obligatorily stored in secretory granules in exocrine cells.
V Colomer, M. Rindler, and A. Lowe (1994)
J. Cell Sci. 107, 2271-2277
   Abstract »    PDF »
GPI-anchored proteins associate to form microdomains during their intracellular transport in Caco-2 cells.
M Garcia, C Mirre, A Quaroni, H Reggio, and A Le Bivic (1993)
J. Cell Sci. 104, 1281-1290
   Abstract »    PDF »
Regulation of cell surface polarity from bacteria to mammals.
W. Nelson (1992)
Science 258, 948-955
   Abstract »    PDF »
Regulation of Epithelial Cell Polarity: A View from the Cell Surface.
W.J. Nelson, R. Wilson, D. Wollner, R. Mays, H. McNeill, and K. Siemers (1992)
Cold Spring Harb Symp Quant Biol 57, 621-630
   Abstract »    PDF »
N-Linked Oligosaccharides on the Meprin A Metalloprotease Are Important for Secretion and Enzymatic Activity, but Not for Apical Targeting.
T. Kadowaki, T. Tsukuba, G. P. Bertenshaw, and J. S. Bond (2000)
J. Biol. Chem. 275, 25577-25584
   Abstract »    Full Text »    PDF »
Regulated Apical Secretion of Zymogens in Rat Pancreas. INVOLVEMENT OF THE GLYCOSYLPHOSPHATIDYLINOSITOL-ANCHORED GLYCOPROTEIN GP-2, THE LECTIN ZG16p, AND CHOLESTEROL-GLYCOSPHINGOLIPID-ENRICHED MICRODOMAINS.
K. Schmidt, M. Schrader, H.-F. Kern, and R. Kleene (2001)
J. Biol. Chem. 276, 14315-14323
   Abstract »    Full Text »    PDF »
Multiple Regions within the Coxsackievirus and Adenovirus Receptor Cytoplasmic Domain Are Required for Basolateral Sorting.
C. J. Cohen, J. Gaetz, T. Ohman, and J. M. Bergelson (2001)
J. Biol. Chem. 276, 25392-25398
   Abstract »    Full Text »    PDF »
Saturation of, and competition for entry into, the apical secretory pathway.
A. D. Marmorstein, K. G. Csaky, J. Baffi, L. Lam, F. Rahaal, and E. Rodriguez-Boulan (2000)
PNAS 97, 3248-3253
   Abstract »    Full Text »    PDF »


To Advertise     Find Products

Science. ISSN 0036-8075 (print), 1095-9203 (online)