Note to users. If you're seeing this message, it means that your browser cannot find this page's style/presentation instructions -- or possibly that you are using a browser that does not support current Web standards. Find out more about why this message is appearing, and what you can do to make your experience of our site the best it can be.


Science 29 September 1989:
Vol. 245. no. 4925, pp. 1463 - 1473
DOI: 10.1126/science.245.4925.1463

Articles

The Photosynthetic Reaction Center from the Purple Bacterium Rhodopseudomonas viridis

JOHANN DEISENHOFER 1 and HARTMUT MICHEL 2

1 Howard Hughes Medical Institute and Department of Biochemistry, University of Texas Southwestern Medical Center at Dallas, 5323 Harry Hines Blvd., Dallas, TX 75235-9050.
2 Max-Planck-Institute for Biophysics, Heinrich-Hoffmann-Strasse 7, D-6000 Frankfurt/M 71, Federal Republic of Germany.

The history and methods of membrane protein crystallization are described. The solution of the structure of the photosynthetic reaction center from the bacterium Rhodopseudomonas viridis is described, and the structure of this membrane protein complex is correlated with its function as a light-driven electron pump across the photosynthetic membrane. Conclusions about the structure of the photosystem II reaction center from plants are drawn, and aspects of membrane protein structure are discussed.


THIS ARTICLE HAS BEEN CITED BY OTHER ARTICLES:
Photoproduction of proton gradients with pi-stacked fluorophore scaffolds in lipid bilayers..
S. Bhosale, A. L. Sisson, P. Talukdar, A. Furstenberg, N. Banerji, E. Vauthey, G. Bollot, J. Mareda, C. Roger, F. Wurthner, et al. (2006)
Science 313, 84-86
   Abstract »    Full Text »    PDF »
Evidence for Electron Equilibrium between the Two Hemes bL in the Dimeric Cytochrome bc1 Complex.
X. Gong, L. Yu, D. Xia, and C.-A. Yu (2005)
J. Biol. Chem. 280, 9251-9257
   Abstract »    Full Text »    PDF »
Mutations within the Putative Membrane-Spanning Domain of the Simian Immunodeficiency Virus Transmembrane Glycoprotein Define the Minimal Requirements for Fusion, Incorporation, and Infectivity.
J. T. West, P. B. Johnston, S. R. Dubay, and E. Hunter (2001)
J. Virol. 75, 9601-9612
   Abstract »    Full Text »    PDF »
Internal packing of helical membrane proteins.
M. Eilers, S. C. Shekar, T. Shieh, S. O. Smith, and P. J. Fleming (2000)
PNAS 97, 5796-5801
   Abstract »    Full Text »    PDF »
A simple method for predicting transmembrane {alpha} helices with better accuracy.
M. M. Gromiha (1999)
Protein Eng. Des. Sel. 12, 557-561
   Abstract »    Full Text »    PDF »
Isolated transmembrane helices arranged across a membrane: computational studies.
V. M. Tseitin and G. V. Nikiforovich (1999)
Protein Eng. Des. Sel. 12, 305-311
   Abstract »    Full Text »    PDF »
Identification of Quinone-binding and Heme-ligating Residues of the Smallest Membrane-anchoring Subunit (QPs3) of Bovine Heart Mitochondrial Succinate:Ubiquinone Reductase.
S. K. Shenoy, L. Yu, and C.-a. Yu (1999)
J. Biol. Chem. 274, 8717-8722
   Abstract »    Full Text »    PDF »
Critical Amino Acid Residues in Transmembrane Span 7 of the Serotonin Transporter Identified by Random Mutagenesis.
K. M. Y. Penado, G. Rudnick, and M. M. Stephan (1998)
J. Biol. Chem. 273, 28098-28106
   Abstract »    Full Text »    PDF »
Cys-scanning mutagenesis: a novel approach to structure–function relationships in polytopic membrane proteins.
S. Frillingos, M. Sahin-tóth, J. Wu, and H. R. Kaback (1998)
FASEB J 12, 1281-1299
   Abstract »    Full Text »
Chemical complementation identifies a proton acceptor for redox-active tyrosine D in photosystem II.
S. Kim, J. Liang, and B. A. Barry (1997)
PNAS 94, 14406-14411
   Abstract »    Full Text »    PDF »
Dimer to Monomer Conversion of the Cytochrome b6 f Complex. CAUSES AND CONSEQUENCES.
C. Breyton, C. Tribet, J. Olive, J.-P. Dubacq, and J.-L. Popot (1997)
J. Biol. Chem. 272, 21892-21900
   Abstract »    Full Text »    PDF »
Lipidic cubic phases: A novel concept for the crystallization of membrane proteins.
E. M. Landau and J. P. Rosenbusch (1996)
PNAS 93, 14532-14535
   Abstract »    Full Text »    PDF »
A Difference Fourier Transform Infrared Spectroscopic Study of Chlorophyll Oxidation in Hydroxylamine-treated Photosystem II.
G. M. MacDonald, J. J. Steenhuis, and B. A. Barry (1995)
J. Biol. Chem. 270, 8420-8428
   Abstract »    Full Text »    PDF »
A Difference Infrared Study of Hydrogen Bonding to the Z Tyrosyl Radical of Photosystem II.
M. T. Bernard, G. M. MacDonald, A. P. Nguyen, R. J. Debus, and B. A. Barry (1995)
J. Biol. Chem. 270, 1589-1594
   Abstract »    Full Text »    PDF »
Effective coupling in biological electron transfer: exponential or complex distance dependence?.
J. Evenson and M Karplus (1993)
Science 262, 1247-1249
   Abstract »    PDF »
The 2.3 angstrom X-ray structure of nitrite reductase from Achromobacter cycloclastes.
J. Godden, S Turley, D. Teller, E. Adman, M. Liu, W. Payne, and J LeGall (1991)
Science 253, 438-442
   Abstract »    PDF »
Partial symmetrization of the photosynthetic reaction center.
S. Robles, J Breton, and D. Youvan (1990)
Science 248, 1402-1405
   Abstract »    PDF »
Transmembrane protein structure: spin labeling of bacteriorhodopsin mutants.
C Altenbach, T Marti, H. Khorana, and W. Hubbell (1990)
Science 248, 1088-1092
   Abstract »    PDF »



To Advertise     Find Products


Science. ISSN 0036-8075 (print), 1095-9203 (online)