Note to users. If you're seeing this message, it means that your browser cannot find this page's style/presentation instructions -- or possibly that you are using a browser that does not support current Web standards. Find out more about why this message is appearing, and what you can do to make your experience of our site the best it can be.

Science 8 September 1989:
Vol. 245. no. 4922, pp. 1115 - 1118
DOI: 10.1126/science.2549640
Prev | Table of Contents | Next

Articles

Science, Vol 245, Issue 4922, 1115-1118
Copyright © 1989 by American Association for the Advancement of Science

articles

Primary structure of the beta subunit of the DHP-sensitive calcium channel from skeletal muscle

P Ruth, A Rohrkasten, M Biel, E Bosse, S Regulla, HE Meyer, V Flockerzi, and F Hofmann

Institut fur Physiologische Chemie, Medizinische Fakultat, Homburg/Saar, Federal Republic of Germany.

Complementary DNAs for the beta subunit of the dihydropyridine-sensitive calcium channel of rabbit skeletal muscle were isolated on the basis of peptide sequences derived from the purified protein. The deduced primary structure is without homology to other known protein sequences and is consistent with the beta subunit being a peripheral membrane protein associated with the cytoplasmic aspect of the sarcolemma. The protein contains sites that might be expected to be preferentially phosphorylated by protein kinase C and guanosine 3',5'-monophosphate-dependent protein kinase. A messenger RNA for this protein appears to be expressed in brain.


THIS ARTICLE HAS BEEN CITED BY OTHER ARTICLES:
Engineering Proteins for Custom Inhibition of CaV Channels.
X. Xu and H. M. Colecraft (2009)
Physiology 24, 210-218
   Abstract »    Full Text »    PDF »
Coupled and Independent Contributions of Residues in IS6 and IIS6 to Activation Gating of CaV1.2.
M. Kudrnac, S. Beyl, A. Hohaus, A. Stary, T. Peterbauer, E. Timin, and S. Hering (2009)
J. Biol. Chem. 284, 12276-12284
   Abstract »    Full Text »    PDF »
Supramolecular Assemblies and Localized Regulation of Voltage-Gated Ion Channels.
S. Dai, D. D. Hall, and J. W. Hell (2009)
Physiol Rev 89, 411-452
   Abstract »    Full Text »    PDF »
Proper Restoration of Excitation-Contraction Coupling in the Dihydropyridine Receptor {beta}1-null Zebrafish Relaxed Is an Exclusive Function of the {beta}1a Subunit.
J. Schredelseker, A. Dayal, T. Schwerte, C. Franzini-Armstrong, and M. Grabner (2009)
J. Biol. Chem. 284, 1242-1251
   Abstract »    Full Text »    PDF »
Role of CaV{beta} Subunits, and Lack of Functional Reserve, in Protein Kinase A Modulation of Cardiac CaV1.2 Channels.
J. Miriyala, T. Nguyen, D. T. Yue, and H. M. Colecraft (2008)
Circ. Res. 102, e54-e64
   Abstract »    Full Text »    PDF »
Mechanism of Cav1.2 channel modulation by the amino terminus of cardiac {beta}2-subunits.
S. Herzig, I. F. Y. Khan, D. Grundemann, J. Matthes, A. Ludwig, G. Michels, U. C. Hoppe, D. Chaudhuri, A. Schwartz, D. T. Yue, et al. (2007)
FASEB J 21, 1527-1538
   Abstract »    Full Text »    PDF »
Probing the Architecture of an L-type Calcium Channel with a Charged Phenylalkylamine: EVIDENCE FOR A WIDELY OPEN PORE AND DRUG TRAPPING.
S. Beyl, E. N. Timin, A. Hohaus, A. Stary, M. Kudrnac, R. H. Guy, and S. Hering (2007)
J. Biol. Chem. 282, 3864-3870
   Abstract »    Full Text »    PDF »
The Role of Voltage-Gated Calcium Channels in Pancreatic {beta}-Cell Physiology and Pathophysiology.
S.-N. Yang and P.-O. Berggren (2006)
Endocr. Rev. 27, 621-676
   Abstract »    Full Text »    PDF »
Autoinhibitory control of the CaV1.2 channel by its proteolytically processed distal C-terminal domain.
J. T. Hulme, V. Yarov-Yarovoy, T. W.-C. Lin, T. Scheuer, and W. A. Catterall (2006)
J. Physiol. 576, 87-102
   Abstract »    Full Text »    PDF »
Overview of Molecular Relationships in the Voltage-Gated Ion Channel Superfamily.
F. H. Yu, V. Yarov-Yarovoy, G. A. Gutman, and W. A. Catterall (2005)
Pharmacol. Rev. 57, 387-395
   Full Text »    PDF »
The {beta}1a subunit is essential for the assembly of dihydropyridine-receptor arrays in skeletal muscle.
J. Schredelseker, V. Di Biase, G. J. Obermair, E. T. Felder, B. E. Flucher, C. Franzini-Armstrong, and M. Grabner (2005)
PNAS 102, 17219-17224
   Abstract »    Full Text »    PDF »
Structural Determinants of L-type Channel Activation in Segment IIS6 Revealed by a Retinal Disorder.
A. Hohaus, S. Beyl, M. Kudrnac, S. Berjukow, E. N. Timin, R. Marksteiner, M. A. Maw, and S. Hering (2005)
J. Biol. Chem. 280, 38471-38477
   Abstract »    Full Text »    PDF »
Molecular Physiology of Cardiac Repolarization.
J. M. Nerbonne and R. S. Kass (2005)
Physiol Rev 85, 1205-1253
   Abstract »    Full Text »    PDF »
Opposite Effects of a Single IIIS5 Mutation on Phenylalkylamine and Dihydropyridine Interaction with L-type Ca2+ Channels.
I. G. Huber, E. Wappl-Kornherr, M. J. Sinnegger-Brauns, J.-C. Hoda, D. Walter-Bastl, and J. Striessnig (2004)
J. Biol. Chem. 279, 55211-55217
   Abstract »    Full Text »    PDF »
New Short Splice Variants of the Human Cardiac Cav{beta}2 Subunit: REDEFINING THE MAJOR FUNCTIONAL MOTIFS IMPLEMENTED IN MODULATION OF THE Cav1.2 CHANNEL.
J. B. Harry, E. Kobrinsky, D. R. Abernethy, and N. M. Soldatov (2004)
J. Biol. Chem. 279, 46367-46372
   Abstract »    Full Text »    PDF »
The VGL-Chanome: A Protein Superfamily Specialized for Electrical Signaling and Ionic Homeostasis.
F. H. Yu and W. A. Catterall (2004)
Sci. STKE 2004, re15
   Abstract »    Full Text »    PDF »
Non-voltage-gated L-type Ca2+ Channels in Human T Cells: PHARMACOLOGY AND MOLECULAR CHARACTERIZATION OF THE MAJOR {alpha} PORE-FORMING AND AUXILIARY {beta}-SUBUNITS.
L. Stokes, J. Gordon, and G. Grafton (2004)
J. Biol. Chem. 279, 19566-19573
   Abstract »    Full Text »    PDF »
The Three-dimensional Structure of the Cardiac L-type Voltage-gated Calcium Channel: COMPARISON WITH THE SKELETAL MUSCLE FORM REVEALS A COMMON ARCHITECTURAL MOTIF.
M.-C. Wang, R. F. Collins, R. C. Ford, N. S. Berrow, A. C. Dolphin, and A. Kitmitto (2004)
J. Biol. Chem. 279, 7159-7168
   Abstract »    Full Text »    PDF »
G Protein Modulation of Voltage-Gated Calcium Channels.
A. C. Dolphin (2003)
Pharmacol. Rev. 55, 607-627
   Abstract »    Full Text »    PDF »
{beta}-Adrenergic regulation requires direct anchoring of PKA to cardiac CaV1.2 channels via a leucine zipper interaction with A kinase-anchoring protein 15.
J. T. Hulme, T. W.-C. Lin, R. E. Westenbroek, T. Scheuer, and W. A. Catterall (2003)
PNAS 100, 13093-13098
   Abstract »    Full Text »    PDF »
Differences in Apparent Pore Sizes of Low and High Voltage-activated Ca2+ Channels.
M. Cataldi, E. Perez-Reyes, and R. W. Tsien (2002)
J. Biol. Chem. 277, 45969-45976
   Abstract »    Full Text »    PDF »
Molecular Cloning and Characterization of the Human Voltage-Gated Calcium Channel alpha 2delta -4 Subunit.
N. Qin, S. Yagel, M.-L. Momplaisir, E. E. Codd, and M. R. D'Andrea (2002)
Mol. Pharmacol. 62, 485-496
   Abstract »    Full Text »    PDF »
Novel functional properties of Ca2+ channel {beta} subunits revealed by their expression in adult rat heart cells.
H. M Colecraft, B. Alseikhan, S. X Takahashi, D. Chaudhuri, S. Mittman, V. Yegnasubramanian, R. S Alvania, D. C Johns, E. Marban, and D. T Yue (2002)
J. Physiol. 541, 435-452
   Abstract »    Full Text »    PDF »
Molecular Determinants of Voltage-dependent Slow Inactivation of the Ca2+ Channel.
C. Shi and N. M. Soldatov (2002)
J. Biol. Chem. 277, 6813-6821
   Abstract »    Full Text »    PDF »
Cloning of a Functional Splice Variant of L-type Calcium Channel beta 2 Subunit from Rat Heart.
Y. Yamada, M. Nagashima, M. Tsutsuura, T. Kobayashi, S. Seki, N. Makita, Y. Horio, and N. Tohse (2001)
J. Biol. Chem. 276, 47163-47170
   Abstract »    Full Text »    PDF »
Potentiation of the Cardiac L-Type Ca2+ Channel ({alpha}1C) by Dihydropyridine Agonist and Strong Depolarization Occur via Distinct Mechanisms.
C. M. Wilkens, M. Grabner, and K. G. Beam (2001)
J. Gen. Physiol. 118, 495-508
   Abstract »    Full Text »    PDF »
Three New Familial Hemiplegic Migraine Mutants Affect P/Q-type Ca2+ Channel Kinetics.
R. L. Kraus, M. J. Sinnegger, A. Koschak, H. Glossmann, S. Stenirri, P. Carrera, and J. Striessnig (2000)
J. Biol. Chem. 275, 9239-9243
   Abstract »    Full Text »    PDF »
Regulation of Cloned Cardiac L-type Calcium Channels by cGMP-dependent Protein Kinase.
L. H. Jiang, D. J. Gawler, N. Hodson, C. J. Milligan, H. A. Pearson, V. Porter, and D. Wray (2000)
J. Biol. Chem. 275, 6135-6143
   Abstract »    Full Text »    PDF »
The molecular biology of invertebrate voltage-gated Ca(2+) channels.
M. Jeziorski, R. Greenberg, and P. Anderson (2000)
J. Exp. Biol. 203, 841-856
   Abstract »    PDF »
Voltage-Gated Ion Channels and Hereditary Disease.
F. Lehmann-Horn and K. Jurkat-Rott (1999)
Physiol Rev 79, 1317-1372
   Abstract »    Full Text »    PDF »
Studies on Maitotoxin-Induced Intracellular Ca2+ Elevation in Chinese Hamster Ovary Cells Stably Transfected with cDNAs Encoding for L-Type Ca2+ Channel Subunits.
M. Cataldi, A. Secondo, A. D'Alessio, M. Taglialatela, F. Hofmann, N. Klugbauer, G. Di Renzo, and L. Annunziato (1999)
J. Pharmacol. Exp. Ther. 290, 725-730
   Abstract »    Full Text »
Current modulation and membrane targeting of the calcium channel {alpha}1C subunit are independent functions of the {beta} subunit.
U. Gerster, B. Neuhuber, K. Groschner, J. Striessnig, and B. E Flucher (1999)
J. Physiol. 517, 353-368
   Abstract »    Full Text »    PDF »
Ca channels in cardiac myocytes: structure and function in Ca influx and intracellular Ca release.
D. M Bers and E. Perez-Reyes (1999)
Cardiovasc Res 42, 339-360
   Abstract »    Full Text »    PDF »
Functional Coupling of Human L-Type Ca2+ Channels and Angiotensin AT1A Receptors Coexpressed in Xenopus laevis Oocytes: Involvement of the Carboxyl-Terminal Ca2+ Sensors.
M. Oz, M. T. Melia, N. M. Soldatov, D. R. Abernethy, and M. Morad (1998)
Mol. Pharmacol. 54, 1106-1112
   Abstract »    Full Text »
Molecular Mechanism of Diltiazem Interaction with L-type Ca2+ Channels.
R. L. Kraus, S. Hering, M. Grabner, D. Ostler, and J. Striessnig (1998)
J. Biol. Chem. 273, 27205-27212
   Abstract »    Full Text »    PDF »
Differential Effects of Ca2+ Channel beta 1a and beta 2a Subunits on Complex Formation with alpha 1S and on Current Expression in tsA201 Cells.
B. Neuhuber, U. Gerster, J. Mitterdorfer, H. Glossmann, and B. E. Flucher (1998)
J. Biol. Chem. 273, 9110-9118
   Abstract »    Full Text »    PDF »
Ca2+-sensitive inactivation of L-type Ca2+ channels depends on multiple cytoplasmic amino acid sequences of the alpha 1C subunit.
R. D. Zuhlke and H. Reuter (1998)
PNAS 95, 3287-3294
   Abstract »    Full Text »    PDF »
Familial Hemiplegic Migraine Mutations Change alpha 1A Ca2+ Channel Kinetics.
R. L. Kraus, M. J. Sinnegger, H. Glossmann, S. Hering, and J. Striessnig (1998)
J. Biol. Chem. 273, 5586-5590
   Abstract »    Full Text »    PDF »
Molecular Determinants of L-type Ca2+ Channel Inactivation. SEGMENT EXCHANGE ANALYSIS OF THE CARBOXYL-TERMINAL CYTOPLASMIC MOTIF ENCODED BY EXONS 40-42 OF THE HUMAN alpha 1C SUBUNIT GENE.
N. M. Soldatov, M. Oz, K. A. O'Brien, D. R. Abernethy, and M. Morad (1998)
J. Biol. Chem. 273, 957-963
   Abstract »    Full Text »    PDF »
Nine L-type Amino Acid Residues Confer Full 1,4-Dihydropyridine Sensitivity to the Neuronal Calcium Channel alpha 1A Subunit. ROLE OF L-TYPE MET1188.
M. J. Sinnegger, Z. Wang, M. Grabner, S. Hering, J. Striessnig, H. Glossmann, and J. Mitterdorfer (1997)
J. Biol. Chem. 272, 27686-27693
   Abstract »    Full Text »    PDF »
Functional Expression and Characterization of Skeletal Muscle Dihydropyridine Receptors in Xenopus Oocytes.
D. Ren and L. M. Hall (1997)
J. Biol. Chem. 272, 22393-22396
   Abstract »    Full Text »    PDF »
beta Subunit Heterogeneity in Neuronal L-type Ca2+ Channels.
M. Pichler, T. N. Cassidy, D. Reimer, H. Haase, R. Kraus, D. Ostler, and J. Striessnig (1997)
J. Biol. Chem. 272, 13877-13882
   Abstract »    Full Text »    PDF »
Preferential Interaction of omega -Conotoxins with Inactivated N-type Ca2+ Channels.
J. W. Stocker, L. Nadasdi, R. W. Aldrich, and R. W. Tsien (1997)
J. Neurosci. 17, 3002-3013
   Abstract »    Full Text »    PDF »
Inhibition of Recombinant Ca2+ Channels by Benzothiazepines and Phenylalkylamines: Class-Specific Pharmacology and Underlying Molecular Determinants.
D. Cai, J. G. Mulle, and D. T. Yue (1997)
Mol. Pharmacol. 51, 872-881
   Abstract »    Full Text »
Regional Expression and Cellular Localization of the alpha 1 and beta  Subunit of High Voltage-Activated Calcium Channels in Rat Brain.
A. Ludwig, V. Flockerzi, and F. Hofmann (1997)
J. Neurosci. 17, 1339-1349
   Abstract »    Full Text »    PDF »
Molecular Structures Involved in L-type Calcium Channel Inactivation. ROLE OF THE CARBOXYL-TERMINAL REGION ENCODED BY EXONS 40-42 IN alpha 1C SUBUNIT IN THE KINETICS AND Ca2+ DEPENDENCE OF INACTIVATION.
N. M. Soldatov, R. D. Zuhlke, A. Bouron, and H. Reuter (1997)
J. Biol. Chem. 272, 3560-3566
   Abstract »    Full Text »    PDF »
Two Amino Acid Residues in the IIIS5 Segment of L-Type Calcium Channels Differentially Contribute to 1,4-Dihydropyridine Sensitivity.
J. Mitterdorfer, Z. Wang, M. J. Sinnegger, S. Hering, J. Striessnig, M. Grabner, and H. Glossmann (1996)
J. Biol. Chem. 271, 30330-30335
   Abstract »    Full Text »    PDF »
Absence of the beta  subunit (cchb1) of the skeletal muscle dihydropyridine receptor alters expression of the alpha 1 subunit and eliminates excitation-contraction coupling.
R. G. Gregg, A. Messing, C. Strube, M. Beurg, R. Moss, M. Behan, M. Sukhareva, S. Haynes, J. A. Powell, R. Coronado, et al. (1996)
PNAS 93, 13961-13966
   Abstract »    Full Text »    PDF »
Identification of Palmitoylation Sites within the L-type Calcium Channel beta 2a Subunit and Effects on Channel Function.
A. J. Chien, K. M. Carr, R. E. Shirokov, E. Rios, and M. M. Hosey (1996)
J. Biol. Chem. 271, 26465-26468
   Abstract »    Full Text »    PDF »
Transfer of High Sensitivity for Benzothiazepines from L-type to Class A (BI) Calcium Channels.
S. Hering, S. Aczel, M. Grabner, F. Doring, S. Berjukow, J. Mitterdorfer, M. J. Sinnegger, J. Striessnig, V. E. Degtiar, Z. Wang, et al. (1996)
J. Biol. Chem. 271, 24471-24475
   Abstract »    Full Text »    PDF »
Transfer of L-type Calcium Channel IVS6 Segment Increases Phenylalkylamine Sensitivity of alpha[IMAGE].
F. Döring, V. E. Degtiar, M. Grabner, Jör. Striessnig, S. Hering, and H. Glossmann (1996)
J. Biol. Chem. 271, 11745-11749
   Abstract »    Full Text »    PDF »
betaSubunit Heterogeneity in N-type Ca[IMAGE] Channels.
V. E. S. Scott, M. De Waard, H. Liu, C. A. Gurnett, D. P. Venzke, V. A. Lennon, and K. P. Campbell (1996)
J. Biol. Chem. 271, 3207-3212
   Abstract »    Full Text »    PDF »
Roles of a Membrane-localized beta Subunit in the Formation and Targeting of Functional L-type Ca[IMAGE] Channels.
A. J. Chien, X. Zhao, R. E. Shirokov, T. S. Puri, C. F. Chang, D. Sun, E. Rios, and M. M. Hosey (1995)
J. Biol. Chem. 270, 30036-30044
   Abstract »    Full Text »    PDF »
Molecular Determinants of Cardiac Ca[IMAGE] Channel Pharmacology.
X. Wei, S. Pan, W. Lang, H. Kim, T. Schneider, E. Perez-Reyes, and L. Birnbaumer (1995)
J. Biol. Chem. 270, 27106-27111
   Abstract »    Full Text »    PDF »
Regulation of Dihydropyridine and Ryanodine Receptor Gene Expression in Skeletal Muscle.
A. Ray, J. Kyselovic, J. J. Leddy, J. T. Wigle, B. J. Jasmin, and B. S. Tuana (1995)
J. Biol. Chem. 270, 25837-25844
   Abstract »    Full Text »    PDF »
Calcium Binding in the Pore of L-type Calcium Channels Modulates High Affinity Dihydropyridine Binding.
B. Z. Peterson and W. A. Catterall (1995)
J. Biol. Chem. 270, 18201-18204
   Abstract »    Full Text »    PDF »
Association of Native Ca[IMAGE] Channel [IMAGE] Subunits with the [IMAGE][IMAGE] Subunit Interaction Domain.
D. R. Witcher, M. D. Waard, H. Liu, M. Pragnell, and K. P. Campbell (1995)
J. Biol. Chem. 270, 18088-18093
   Abstract »    Full Text »    PDF »
Properties of the [IMAGE][IMAGE]-[IMAGE] Anchoring Site in Voltage-dependent Ca[IMAGE] Channels.
M. D. Waard, D. R. Witcher, M. Pragnell, H. Liu, and K. P. Campbell (1995)
J. Biol. Chem. 270, 12056-12064
   Abstract »    Full Text »    PDF »
Different Voltage-dependent Inhibition by Dihydropyridines of Human Ca[IMAGE] Channel Splice Variants.
N. M. Soldatov, A. Bouron, and H. Reuter (1995)
J. Biol. Chem. 270, 10540-10543
   Abstract »    Full Text »    PDF »
Stable Expression and Coupling of Cardiac L-Type Ca2+ Channels With ß1-Adrenoceptors.
A. Yatani, M. Wakamori, T. Niidome, S. Yamamoto, I. Tanaka, Y. Mori, K. Katayama, and S. Green (1995)
Circ. Res. 76, 335-342
   Abstract »    Full Text »
Co-localization of 1,4-dihydropyridine receptor alpha 2/delta subunit and N-CAM during early myogenesis in vitro.
S. Vandaele and F Rieger (1994)
J. Cell Sci. 107, 1217-1227
   Abstract »    PDF »
Cardiac Ion Channels.
A. M. Katz (1993)
N. Engl. J. Med. 328, 1244-1251
   Full Text »
Functional subunit structure of voltage-gated calcium channels.
W. Catterall (1991)
Science 253, 1499-1500
   PDF »
The roles of the subunits in the function of the calcium channel.
D Singer, M Biel, I Lotan, V Flockerzi, F Hofmann, and N Dascal (1991)
Science 253, 1553-1557
   Abstract »    PDF »
Primary structure of the gamma subunit of the DHP-sensitive calcium channel from skeletal muscle.
S. Jay, S. Ellis, A. McCue, M. Williams, T. Vedvick, M. Harpold, and K. Campbell (1990)
Science 248, 490-492
   Abstract »    PDF »
Ca2+-sensitive Inactivation and Facilitation of L-type Ca2+ Channels Both Depend on Specific Amino Acid Residues in a Consensus Calmodulin-binding Motif in thealpha 1C subunit.
R. D. Zuhlke, G. S. Pitt, R. W. Tsien, and H. Reuter (2000)
J. Biol. Chem. 275, 21121-21129
   Abstract »    Full Text »    PDF »
Mechanism of Dihydropyridine Interaction with Critical Binding Residues of L-type Ca2+ Channel alpha 1 Subunits.
E. Wappl, J. Mitterdorfer, H. Glossmann, and J. Striessnig (2001)
J. Biol. Chem. 276, 12730-12735
   Abstract »    Full Text »    PDF »
Molecular Basis of Calmodulin Tethering and Ca2+-dependent Inactivation of L-type Ca2+ Channels.
G. S. Pitt, R. D. Zuhlke, A. Hudmon, H. Schulman, H. Reuter, and R. W. Tsien (2001)
J. Biol. Chem. 276, 30794-30802
   Abstract »    Full Text »    PDF »


To Advertise     Find Products

Science. ISSN 0036-8075 (print), 1095-9203 (online)