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Science 25 August 1989:
Vol. 245. no. 4920, pp. 855 - 857
DOI: 10.1126/science.2528207
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Articles

Science, Vol 245, Issue 4920, 855-857
Copyright © 1989 by American Association for the Advancement of Science

articles

Osteoclastic bone resorption by a polarized vacuolar proton pump

HC Blair, SL Teitelbaum, R Ghiselli, and S Gluck

Department of Pathology, Jewish Hospital, Washington University Medical Center, St. Louis, MO 63110.

Bone resorption depends on the formation, by osteoclasts, of an acidic extracellular compartment wherein matrix is degraded. The mechanism by which osteoclasts transport protons into that resorptive microenvironment was identified by means of adenosine triphosphate-dependent weak base accumulation in isolated osteoclast membrane vesicles, which exhibited substrate and inhibition properties characteristic of the vacuolar, electrogenic H+-transporting adenosine triphosphatase (H+-ATPase). Identify of the proton pump was confirmed by immunoblot of osteoclast membrane proteins probed with antibody to vacuolar H+-ATPase isolated from bovine kidney. The osteoclast's H+-ATPase was immunocytochemically localized to the cell-bone attachment site. Immunoelectron microscopy showed that the H+-ATPase was present in the ruffled membrane, the resorptive organ of the cell.


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