Note to users. If you're seeing this message, it means that your browser cannot find this page's style/presentation instructions -- or possibly that you are using a browser that does not support current Web standards. Find out more about why this message is appearing, and what you can do to make your experience of our site the best it can be.

Science 21 July 1989:
Vol. 245. no. 4915, pp. 295 - 297
DOI: 10.1126/science.2665080
Prev | Table of Contents | Next

Articles

Science, Vol 245, Issue 4915, 295-297
Copyright © 1989 by American Association for the Advancement of Science

articles

Localization of the pancreatic beta cell glucose transporter to specific plasma membrane domains

L Orci, B Thorens, M Ravazzola, and HF Lodish

Institute of Histology and Embryology, University of Geneva Medical School, Switzerland.

Immunocytochemical techniques revealed that the "liver-type" glucose transporter is present in the insulin-producing beta cells of rat pancreatic islets but not in other islet endocrine cells. Ultrastructural analysis of the transporter by the protein A-gold technique showed that it is restricted to certain domains of the plasma membrane, its density being sixfold higher in microvilli facing adjacent endocrine cells than in the flat regions of the plasma membrane. These results support a possible role for this glucose transporter in glucose sensing by beta cells and provide evidence that these cells are polarized.


THIS ARTICLE HAS BEEN CITED BY OTHER ARTICLES:
Facilitative Glucose Transporter 9 Expression Affects Glucose Sensing in Pancreatic {beta}-Cells.
S. A. Evans, M. Doblado, M. M. Chi, J. A. Corbett, and K. H. Moley (2009)
Endocrinology 150, 5302-5310
   Abstract »    Full Text »    PDF »
GLUT2 mutations, translocation, and receptor function in diet sugar managing.
A. Leturque, E. Brot-Laroche, and M. Le Gall (2009)
Am J Physiol Endocrinol Metab 296, E985-E992
   Abstract »    Full Text »    PDF »
Evidence of Diminished Glucose Stimulation and Endoplasmic Reticulum Function in Nonoscillatory Pancreatic Islets.
P. Jahanshahi, R. Wu, J. D. Carter, and C. S. Nunemaker (2009)
Endocrinology 150, 607-615
   Abstract »    Full Text »    PDF »
Alterations in net glucose uptake and in the pancreatic B-cell GLUT2 transporter induced by diazoxide and by secretory stimuli.
L Zhao, Z Li, M Kullin, L A H Borg, and F A Karlsson (2005)
J. Endocrinol. 185, 291-299
   Abstract »    Full Text »    PDF »
Role of microvillar cell surfaces in the regulation of glucose uptake and organization of energy metabolism.
K. Lange (2002)
Am J Physiol Cell Physiol 282, C1-C26
   Abstract »    Full Text »    PDF »
Evidence That Extrapancreatic GLUT2-Dependent Glucose Sensors Control Glucagon Secretion.
R. Burcelin and B. Thorens (2001)
Diabetes 50, 1282-1289
   Abstract »    Full Text »
Expression and Function of {alpha}v{beta}3 and {alpha}v{beta}5 Integrins in the Developing Pancreas: Roles in the Adhesion and Migration of Putative Endocrine Progenitor Cells.
V. Cirulli, G. M. Beattie, G. Klier, M. Ellisman, C. Ricordi, V. Quaranta, F. Frasier, J. K. Ishii, A. Hayek, and D. R. Salomon (2000)
J. Cell Biol. 150, 1445-1460
   Abstract »    Full Text »    PDF »
Islet amyloid polypeptide (amylin)-deficient mice develop a more severe form of alloxan-induced diabetes.
H. Mulder, S. Gebre-Medhin, C. Betsholtz, F. Sundler, and B. Ahren (2000)
Am J Physiol Endocrinol Metab 278, E684-E691
   Abstract »    Full Text »    PDF »
Persistent expression of HNF6 in islet endocrine cells causes disrupted islet architecture and loss of beta cell function.
M Gannon, M. Ray, K Van Zee, F Rausa, R. Costa, and C. Wright (2000)
Development 127, 2883-2895
   Abstract »    PDF »
Comparison of the effects of controlled-rate cryopreservation and vitrification on 2-cell mouse embryos and their subsequent development.
H. Uechi, O. Tsutsumi,3, Y. Morita, Y. Takai, and Y. Taketani (1999)
Hum. Reprod. 14, 2827-2832
   Abstract »    Full Text »    PDF »
Overexpression of leptin receptors in pancreatic islets of Zucker diabetic fatty rats restores GLUT-2, glucokinase, and glucose-stimulated insulin secretion.
M.-Y. Wang, K. Koyama, M. Shimabukuro, D. Mangelsdorf, C. B. Newgard, and R. H. Unger (1998)
PNAS 95, 11921-11926
   Abstract »    Full Text »    PDF »
IB1, a JIP-1-related Nuclear Protein Present in Insulin-secreting Cells.
C. Bonny, P. Nicod, and G. Waeber (1998)
J. Biol. Chem. 273, 1843-1846
   Abstract »    Full Text »    PDF »
Differences in Glucose Transporter Gene Expression between Rat Pancreatic [IMAGE]- and [IMAGE]-Cells Are Correlated to Differences in Glucose Transport but Not in Glucose Utilization.
H. Heimberg, A. D. Vos, D. Pipeleers, B. Thorens, and F. Schuit (1995)
J. Biol. Chem. 270, 8971-8975
   Abstract »    Full Text »    PDF »
Diabetic hyperglycemia: link to impaired glucose transport in pancreatic beta cells.
R. Unger (1991)
Science 251, 1200-1205
   Abstract »    PDF »
Underexpression of beta cell high Km glucose transporters in noninsulin-dependent diabetes.
J. Johnson, A Ogawa, L Chen, L Orci, C. Newgard, T Alam, and R. Unger (1990)
Science 250, 546-549
   Abstract »    PDF »


To Advertise     Find Products

Science. ISSN 0036-8075 (print), 1095-9203 (online)