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Science 14 July 1989:
Vol. 245. no. 4914, pp. 160 - 164
DOI: 10.1126/science.2665076

Articles

Science, Vol 245, Issue 4914, 160-164
Copyright © 1989 by American Association for the Advancement of Science


articles

DNA mismatch correction in a defined system

RS Lahue, KG Au, and P Modrich

Department of Biochemistry, Duke University Medical Center, Durham, NC 27710.

DNA mismatch correction is a strand-specific process involving recognition of noncomplementary Watson-Crick nucleotide pairs and participation of widely separated DNA sites. The Escherichia coli methyl-directed reaction has been reconstituted in a purified system consisting of MutH, MutL, and MutS proteins, DNA helicase II, single-strand DNA binding protein, DNA polymerase III holoenzyme, exonuclease I, DNA ligase, along with ATP (adenosine triphosphate), and the four deoxynucleoside triphosphates. This set of proteins can process seven of the eight base-base mismatches in a strand-specific reaction that is directed by the state of methylation of a single d(GATC) sequence located 1 kilobase from the mispair.


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   Abstract »    Full Text »
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   Abstract »    Full Text »    PDF »
Saccharomyces cerevisiae Msh2p and Msh6p ATPase Activities Are Both Required during Mismatch Repair.
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   Abstract »    Full Text »    PDF »
Mismatch-, MutS-, MutL-, and Helicase II-dependent Unwinding from the Single-strand Break of an Incised Heteroduplex.
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J. Biol. Chem. 273, 9202-9207
   Abstract »    Full Text »    PDF »
The Chromosome Bias of Misincorporations During Double-Strand Break Repair Is Not Altered in Mismatch Repair–Defective Strains of Saccharomyces cerevisiae.
C. B. McGill, S. L. Holbeck, and J. N. Strathern (1998)
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   Abstract »    Full Text »    PDF »
DNA Mismatch Repair Catalyzed by Extracts of Mitotic, Postmitotic, and Senescent Drosophila Tissues and Involvement of mei-9 Gene Function for Full Activity.
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   Abstract »    Full Text »
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Functional Properties of Replication Fork Assemblies Established by the Bacteriophage lambda  O and P Replication Proteins.
K. M. Stephens and R. McMacken (1997)
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Toxicol Pathol 25, 674-696
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Methyl-directed Repair of Mismatched Small Heterologous Sequences in Cell Extracts from Escherichia coli.
W.-h. Fang, J.-Y. Wu, and M.-J. Su (1997)
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Mutation of a Highly Conserved Arginine in Motif IV of Escherichia coli DNA Helicase II Results in an ATP-binding Defect.
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A Point Mutation in Escherichia coli DNA Helicase II Renders the Enzyme Nonfunctional in Two DNA Repair Pathways. EVIDENCE FOR INITIATION OF UNWINDING FROM A NICK IN VIVO.
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Biochemistry and genetics of eukaryotic mismatch repair..
R Kolodner (1996)
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Identification and Characterization of a Thermostable MutS Homolog from Thermus aquaticus.
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DnaX Complex of Escherichia coli DNA Polymerase III Holoenzyme.
H. G. Dallmann, R. L. Thimmig, and C. S. McHenry (1995)
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Appropriate partners make good matches.
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HU Protein of Escherichia coli Binds Specifically to DNA That Contains Single-strand Breaks or Gaps.
B. Castaing, C. Zelwer, J. Laval, and S. Boiteux (1995)
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A role for exonuclease I from S. pombe in mutation avoidance and mismatch correction.
P Szankasi and G. Smith (1995)
Science 267, 1166-1169
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The Saccharomyces cerevisiae Msh2 protein specifically binds to duplex oligonucleotides containing mismatched DNA base pairs and insertions..
E Alani, N W Chi, and R Kolodner (1995)
Genes & Dev. 9, 234-247
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Greater susceptibility to mutations in lagging strand of DNA replication in Escherichia coli than in leading strand.
X Veaute and R. Fuchs (1993)
Science 261, 598-600
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Mismatch Repair and Genetic Stability in Human Cells.
W.-H. Fang, G.-M. Li, M. Longley, J. Holmes, W. Thilly, and P. Modrich (1993)
Cold Spring Harb Symp Quant Biol 58, 597-603
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Escherichia coli MutL Loads DNA Helicase II onto DNA.
L. E. Mechanic, B. A. Frankel, and S. W. Matson (2000)
J. Biol. Chem. 275, 38337-38346
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Excision of 3' Termini by the Trex1 and TREX2 3'right-arrow5' Exonucleases. CHARACTERIZATION OF THE RECOMBINANT PROTEINS.
D. J. Mazur and F. W. Perrino (2001)
J. Biol. Chem. 276, 17022-17029
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The Interaction of DNA Mismatch Repair Proteins with Human Exonuclease I.
C. Schmutte, M. M. Sadoff, K.-S. Shim, S. Acharya, and R. Fishel (2001)
J. Biol. Chem. 276, 33011-33018
   Abstract »    Full Text »    PDF »
Redundant Exonuclease Involvement in Escherichia coli Methyl-directed Mismatch Repair.
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