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Science 23 June 1989:
Vol. 244. no. 4911, pp. 1472 - 1475
DOI: 10.1126/science.2472008
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Articles

Science, Vol 244, Issue 4911, 1472-1475
Copyright © 1989 by American Association for the Advancement of Science

articles

Persistence of abnormal chloride conductance regulation in transformed cystic fibrosis epithelia

AM Jetten, Yankaskas JR, MJ Stutts, NJ Willumsen, and RC Boucher

National Institute of Environmental Health Sciences, Research Triangle Park, NC 27709.

An airway epithelial cell line (CF/T43) was developed by infecting cultured airway epithelial cells from patients with cystic fibrosis (CF) with the pZIPneoSV(X)1/SV40T retrovirus and selecting for G418 resistance and ion transport properties. The distinctive chloride secretory phenotypes of the CF cell line CF/T43 and a normal cell line (NL/T4) were not perturbed by SV40T-induced cell transformation. Epithelial cell lines generated from CF cells with the SV40T gene can be used to test candidate CF genes and to evaluate the molecular mechanisms responsible for the CF phenotype.


THIS ARTICLE HAS BEEN CITED BY OTHER ARTICLES:
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Am J Physiol Cell Physiol 280, C228-C236
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Reduced Interleukin-8 Production by Cystic Fibrosis Airway Epithelial Cells.
A.R. D. Massengale, F. Quinn Jr., J. Yankaskas, D. Weissman, W. Thomas McClellan, C. Cuff, and S. C. Aronoff (1999)
Am. J. Respir. Cell Mol. Biol. 20, 1073-1080
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Enhanced Efficiency of Lactosylated Poly-L-Lysine-Mediated Gene Transfer into Cystic Fibrosis Airway Epithelial Cells.
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CFTR Is a Conductance Regulator as well as a Chloride Channel.
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Factors Influencing Adeno-Associated Virus-Mediated Gene Transfer to Human Cystic Fibrosis Airway Epithelial Cells: Comparison with Adenovirus Vectors.
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Morphological and functional characterization of a conditionally immortalized collecting tubule cell line.
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Bioelectric Properties of Gerbil Middle Ear Epithelia.
L. M. de Serres, M. R. Van Scott, H. C. Pillsbury, and J. Prazma (1991)
Arch Otolaryngol Head Neck Surg 117, 416-421
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Cell cycle dependence of chloride permeability in normal and cystic fibrosis lymphocytes.
J. Bubien, K. Kirk, T. Rado, and R. Frizzell (1990)
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Identification of the cystic fibrosis gene: chromosome walking and jumping.
J. Rommens, M. Iannuzzi, B Kerem, M. Drumm, G Melmer, M Dean, R Rozmahel, J. Cole, D Kennedy, N Hidaka, et al. (1989)
Science 245, 1059-1065
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The basic defect in cystic fibrosis.
I. Levitan (1989)
Science 244, 1423
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