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Science 12 May 1989:
Vol. 244. no. 4905, pp. 692 - 694
DOI: 10.1126/science.2470151
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Articles

Science, Vol 244, Issue 4905, 692-694
Copyright © 1989 by American Association for the Advancement of Science

articles

Stereochemical course of catalysis by the Tetrahymena ribozyme

J Rajagopal, JA Doudna, and JW Szostak

Department of Molecular Biology, Massachusetts General Hospital, Boston 02114.

The group I intron from Tetrahymena catalyzes phosphodiester transfer reactions on various RNA substrates. A modified RNA substrate with a phosphorothioate group in one stereoisomeric form at the site of reaction was synthesized in order to determine the stereochemical course of an RNA-catalyzed reaction. The reaction product was digested with a stereospecific nuclease to determine the configuration of the product phosphorothioate. The reaction occurs with inversion of configuration at phosphorus, implying an in-line pathway for the reaction.


THIS ARTICLE HAS BEEN CITED BY OTHER ARTICLES:
Inaugural Article: Biography of Jennifer A. Doudna.
M. Marino (2004)
PNAS 101, 16987-16989
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Synthesis of phosphorothioamidites derived from 3'-thio-3'-deoxythymidine and 3'-thio-2',3'-dideoxycytidine and the automated synthesis of oligodeoxynucleotides containing a 3'-S-phosphorothiolate linkage.
G. Sabbagh, K. J. Fettes, R. Gosain, I. A. O'Neil, and R. Cosstick (2004)
Nucleic Acids Res. 32, 495-501
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SURVEY AND SUMMARY: Recent advances in the elucidation of the mechanisms of action of ribozymes.
Y. Takagi, M. Warashina, W. J. Stec, K. Yoshinari, and K. Taira (2001)
Nucleic Acids Res. 29, 1815-1834
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The stereochemical course of group II intron self-splicing.
R. Padgett, M Podar, S. Boulanger, and P. Perlman (1994)
Science 266, 1685-1688
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Aminoacyl esterase activity of the Tetrahymena ribozyme.
J. Piccirilli, T. McConnell, A. Zaug, H. Noller, and T. Cech (1992)
Science 256, 1420-1424
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