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Science 28 April 1989:
Vol. 244. no. 4903, pp. 437 - 440
DOI: 10.1126/science.2717936
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Articles

Science, Vol 244, Issue 4903, 437-440
Copyright © 1989 by American Association for the Advancement of Science

articles

Catalytic antibodies with lipase activity and R or S substrate selectivity

KD Janda, SJ Benkovic, and RA Lerner

Department of Molecular Biology, Research Institute of Scripps Clinic, La Jolla, CA 92037.

The specific hydrolysis of unactivated esters bearing an R or S enantiomeric alcohol has been achieved by two separate classes of catalytic antibodies induced to bind either the R or S substrates. The antibodies exhibit rate accelerations (10(3) to 10(5] above background hydrolysis that, coupled with their antipodal specificity, provide a novel set of reagents for use in synthesis.


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