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Science 21 April 1989:
Vol. 244. no. 4902, pp. 334 - 337
DOI: 10.1126/science.2523561
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Articles

Science, Vol 244, Issue 4902, 334-337
Copyright © 1989 by American Association for the Advancement of Science

articles

Expression of high-affinity binding of human immunoglobulin E by transfected cells

L Miller, U Blank, H Metzger, and JP Kinet

Section on Chemical Immunology, National Institute of Arthritis and Musculoskeletal and Skin Diseases, Bethesda, MD 20892.

The receptor with high affinity for immunoglobulin E (IgE) on mast cells and basophils is critical in initiating allergic reactions. It is composed of an IgE-binding alpha subunit, a beta subunit, and two gamma subunits. The human alpha subunit was expressed on transfected cells in the presence of rat beta and gamma subunits or in the presence of the gamma subunit alone. The IgE binding properties of the expressed human alpha were characteristic of receptors on normal human cells. These results now permit a systematic analysis of human IgE binding and a search for therapeutically useful inhibitors of that binding.


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