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Science 14 April 1989:
Vol. 244. no. 4901, pp. 221 - 224
DOI: 10.1126/science.2539643
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Articles

Science, Vol 244, Issue 4901, 221-224
Copyright © 1989 by American Association for the Advancement of Science

articles

Expression of a cloned rat brain potassium channel in Xenopus oocytes

MJ Christie, JP Adelman, J Douglass, and RA North

Vollum Institute, Oregon Health Sciences University, Portland 97201.

Potassium channels are ubiquitous membrane proteins with essential roles in nervous tissue, but little is known about the relation between their function and their molecular structure. A complementary DNA library was made from rat hippocampus, and a complementary DNA clone (RBK-1) was isolated. The predicted sequence of the 495-amino acid protein is homologous to potassium channel proteins encoded by the Shaker locus of Drosophila and differs by only three amino acids from the expected product of a mouse clone MBK-1. Messenger RNA transcribed from RBK-1 in vitro directed the expression of potassium channels when it was injected into Xenopus oocytes. The potassium current through the expressed channels resembles both the transient (or A) and the delayed rectifier currents reported in mammalian neurons and is sensitive to both 4-aminopyridine and tetraethylammonium.


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