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Science 7 April 1989:
Vol. 244. no. 4900, pp. 79 - 82
DOI: 10.1126/science.2704991
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Articles

Science, Vol 244, Issue 4900, 79-82
Copyright © 1989 by American Association for the Advancement of Science

articles

Odor-induced membrane currents in vertebrate-olfactory receptor neurons

S Firestein and F Werblin

Graduate Group in Neurobiology, University of California, Berkeley 94720.

In olfactory receptor neurons, odor molecules cause a depolarization that leads to action potential generation. Underlying the depolarization is an ionic current that is the earliest electrical event in the transduction process. In two preparations, olfactory receptor neurons were voltage-clamped and stimulated with odors and this generator current was measured. In addition, a method was developed to estimate the time course and absolute concentration of odorants delivered to the receptor sites. With this method, olfactory neurons were found to have relatively high stimulus thresholds, steep dose-response relations, long latencies, and an apparent requirement for cooperativity at one or more steps in the pathway from odorant binding to activation of the generator current.


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