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Science 17 March 1989:
Vol. 243. no. 4897, pp. 1483 - 1485
DOI: 10.1126/science.2467379
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Articles

Science, Vol 243, Issue 4897, 1483-1485
Copyright © 1989 by American Association for the Advancement of Science

articles

Fluid flow stimulates tissue plasminogen activator secretion by cultured human endothelial cells

SL Diamond, SG Eskin, and LV McIntire

Rice University, Biomedical Engineering Laboratory, Houston, TX 77251.

Wall shear stress generated by blood flow may regulate the expression of fibrinolytic proteins by endothelial cells. Tissue plasminogen activator (tPA) and plasminogen activator inhibitor, type 1 (PAI-1) secretion by cultured human endothelial cells were not affected by exposure to venous shear stress (4 dynes/cm2). However, at arterial shear stresses of 15 and 25 dynes/cm2, the tPA secretion rate was 2.1 and 3.0 times greater, respectively, than the basal tPA secretion rate. PAI-1 secretion was unaffected by shear stress over the entire physiological range.


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