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Science 17 March 1989: Vol. 243. no. 4897, pp. 1464 - 1466 DOI: 10.1126/science.2648569
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Articles
Science, Vol 243, Issue 4897, 1464-1466
Copyright © 1989 by American Association for the Advancement of Science
The neutrophil-activating protein (NAP-1) is also chemotactic for T lymphocytes
CG Larsen,
AO Anderson,
E Appella,
JJ Oppenheim,
and
K Matsushima
Laboratory of Molecular Immunoregulation, National Cancer Institute, Frederick, MD 21701.
T lymphocyte chemotactic factor (TCF) was purified to homogeneity from the conditioned media of phytohemagglutinin-stimulated human blood mononuclear leukocytes by a sequence of chromatography procedures. The amino-terminal amino acid sequence of the purified TCF showed identity with neutrophil-activating protein (NAP-1). Both TCF and recombinant NAP-1 (rNAP-1) were chemotactic for neutrophils and T lymphocytes in vitro supporting the identity of TCF with NAP-1. Injection of rNAP-1 into lymphatic drainage areas of lymph nodes in Fisher rats caused accelerated emigration of only lymphocytes in high endothelial venules. Intradermal injection of rNAP-1 caused dose-dependent accumulation of neutrophils and lymphocytes.
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