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Science 10 March 1989:
Vol. 243. no. 4896, pp. 1363 - 1366
DOI: 10.1126/science.2646717
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Articles

Science, Vol 243, Issue 4896, 1363-1366
Copyright © 1989 by American Association for the Advancement of Science

articles

Nucleotides in yeast tRNAPhe required for the specific recognition by its cognate synthetase

Sampson JR, AB DiRenzo, LS Behlen, and OC Uhlenbeck

Department of Chemistry and Biochemistry, University of Colorado, Boulder 80309.

An analysis of the aminoacylation kinetics of unmodified yeast tRNAPhe mutants revealed that five single-stranded nucleotides are important for its recognition by yeast phenylalanyl-tRNA synthetase, provided they were positioned correctly in a properly folded tRNA structure. When four other tRNAs were changed to have these five nucleotides, they became near-normal substrates for the enzyme.


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