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Science 10 March 1989:
Vol. 243. no. 4896, pp. 1351 - 1354
DOI: 10.1126/science.243.4896.1351
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Articles

A Dwarf Mutant of Arabidopsis Generated by T-DNA Insertion Mutagenesis

Kenneth A. Feldmann 1, M. David Marks 2, Michael L. Christianson 3, and Ralph S. Quatrano 1

1 E. I. du Pont de Nemours & Co., Wilmington, DE 19880-0402
2 School of Biological Sciences, University of Nebraska, Lincoln, NE 68588
3 Sandoz Crop Protection, Palo Alto, CA 94304

Most plant genes that control complex traits of tissues, organs, and whole plants are uncharacterized. Plant height, structure of reproductive organs, seed development and germination, for example, are traits of great agronomic importance. However, in the absence of knowledge of the gene products, current molecular approaches to isolate these important genes are limited. Infection of germinatng seeds of Arabidopsis thaliana with Agrobacterium results in transformed lines in which the integrated T-DNA from Agrobacterium and its associated kanamycin-resistance trait cosegregate with stable, phenotypic alterations. A survey of 136 transformed lines produced plants segregating in a manner consistent with Mendelian predictions for phenotypes altered in height, flower structure, trichomes, gametogenesis, embryogenesis, and seedling development. This report is the characterization of a dwarf mutant in which the phenotype is inherited as a single recessive nuclear mutation that cosegregates with both the kanamycin-resistance trait and the T-DNA insert.

Submitted on September 30, 1988
Accepted on December 20, 1988


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Identification of a mobile endogenous transposon in Arabidopsis thaliana.
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Genetically Engineering Plants for Crop Improvement.
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Science. ISSN 0036-8075 (print), 1095-9203 (online)