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Science 3 March 1989: Vol. 243. no. 4895, pp. 1160 - 1165 DOI: 10.1126/science.2466335
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Articles
Science, Vol 243, Issue 4895, 1160-1165
Copyright © 1989 by American Association for the Advancement of Science
Endothelial leukocyte adhesion molecule 1: an inducible receptor for neutrophils related to complement regulatory proteins and lectins
MP Bevilacqua,
S Stengelin,
MA Gimbrone Jr,
and
B Seed
Department of Pathology, Brigham and Women's Hospital, Boston, MA 02115.
Focal adhesion of leukocytes to the blood vessel lining is a key step in inflammation and certain vascular disease processes. Endothelial leukocyte adhesion molecule-1 (ELAM-1), a cell surface glycoprotein expressed by cytokine-activated endothelium, mediates the adhesion of blood neutrophils. A full-length complementary DNA (cDNA) for ELAM-1 has now been isolated by transient expression in COS cells. Cells transfected with the ELAM-1 clone express a surface structure recognized by two ELAM-1 specific monoclonal antibodies (H4/18 and H18/7) and support the adhesion of isolated human neutrophils and the promyelocytic cell line HL-60. Expression of ELAM-1 transcripts in cultured human endothelial cells is induced by cytokines, reaching a maximum at 2 to 4 hours and decaying by 24 hours; cell surface expression of ELAM-1 protein parallels that of the mRNA. The primary sequence of ELAM-1 predicts an amino-terminal lectin-like domain, an EGF domain, and six tandem repetitive motifs (about 60 amino acids each) related to those found in complement regulatory proteins. A similar domain structure is also found in the MEL-14 lymphocyte cell surface homing receptor, and in granule-membrane protein 140, a membrane glycoprotein of platelet and endothelial secretory granules that can be rapidly mobilized (less than 5 minutes) to the cell surface by thrombin and other stimuli. Thus, ELAM-1 may be a member of a nascent gene family of cell surface molecules involved in the regulation of inflammatory and immunological events at the interface of vessel wall and blood.
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- A. J. Naiyer, D.-Y. Jo, J. Ahn, R. Mohle, M. Peichev, G. Lam, R. L. Silverstein, M. A.S. Moore, and S. Rafii (1999)
Blood
94, 4011-4019
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- Skeletal muscle reperfusion injury is mediated by neutrophils and the complement membrane attack complex.
- C. Kyriakides, W. Austen Jr., Y. Wang, J. Favuzza, L. Kobzik, F. D. Moore Jr., and H. B. Hechtman (1999)
Am J Physiol Cell Physiol
277, C1263-C1268
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- Divergent Inducible Expression of P-Selectin and E-Selectin in Mice and Primates.
- L. Yao, H. Setiadi, L. Xia, Z. Laszik, F. B. Taylor, and R. P. McEver (1999)
Blood
94, 3820-3828
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- Plasmodium falciparum domain mediating adhesion to chondroitin sulfate A: A receptor for human placental infection.
- P. A. Buffet, B. Gamain, C. Scheidig, D. Baruch, J. D. Smith, R. Hernandez-Rivas, B. Pouvelle, S. Oishi, N. Fujii, T. Fusai, et al. (1999)
PNAS
96, 12743-12748
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- Comment on Alteration of monocyte function in patients with growth hormone deficiency: Effect of substitutive GH therapy.
- T. Elhadd, T. Abdu, R. Clayton, and J. Belch (1999)
J. Clin. Endocrinol. Metab.
84, 3405a-3406
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- Adhesion Molecules in Cerebrovascular Diseases : Evidence for an Inflammatory Endothelial Activation in Cerebral Large- and Small-Vessel Disease.
- K. Fassbender, T. Bertsch, O. Mielke, F. Muhlhauser, and M. Hennerici (1999)
Stroke
30, 1647-1650
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- Loss of Endothelial Surface Expression of E-Selectin in a Patient With Recurrent Infections.
- H. M. DeLisser, M. Christofidou-Solomidou, J. Sun, M. T. Nakada, and K. E. Sullivan (1999)
Blood
94, 884-894
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- Chemokines in Lung Injury: Thomas A. Neff Lecture.
- R. M. Strieter, S. L. Kunkel, M. P. Keane, and T. J. Standiford (1999)
Chest
116
, 103S-110S
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- Noninflammatory Expression of E-Selectin Is Regulated by Cell Growth.
- J. Luo, G. Paranya, and J. Bischoff (1999)
Blood
93, 3785-3791
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- Overexpression of A1, an NF-kappa B-Inducible Anti-Apoptotic Bcl Gene, Inhibits Endothelial Cell Activation.
- D. M. Stroka, A. Z. Badrichani, F. H. Bach, and C. Ferran (1999)
Blood
93, 3803-3810
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- Endothelial Cells Undergoing Apoptosis Become Proadhesive for Nonactivated Platelets.
- T. Bombeli, B. R. Schwartz, and J. M. Harlan (1999)
Blood
93, 3831-3838
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- Pediatric AIDS-Associated Lymphocytic Interstitial Pneumonia and Pulmonary Arterio-Occlusive Disease : Role of VCAM-1/VLA-4 Adhesion Pathway and Human Herpesviruses.
- S. J. Brodie, C. de la Rosa, J. G. Howe, J. Crouch, W. D. Travis, and K. Diem (1999)
Am. J. Pathol.
154, 1453-1464
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- Synergistic Effects of Interleukin-4 or Interleukin-13 and Tumor Necrosis Factor-alpha on Eosinophil Activation In Vitro.
- W. Luttmann, T. Matthiesen, H. Matthys, and J. C. Virchow Jr. (1999)
Am. J. Respir. Cell Mol. Biol.
20, 474-480
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- Activated T Cells Acquire Endothelial Cell Surface Determinants During Transendothelial Migration.
- R. I. Brezinschek, N. Oppenheimer-Marks, and P. E. Lipsky (1999)
J. Immunol.
162, 1677-1684
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- Endothelial Cells Express a Novel, Tumor Necrosis Factor-alpha -regulated Variant of HOXA9.
- C. V. Patel, R. Sharangpani, S. Bandyopadhyay, and P. E. DiCorleto (1999)
J. Biol. Chem.
274, 1415-1422
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- Selective Requirements for Leukocyte Adhesion Molecules in Models of Acute and Chronic Cutaneous Inflammation: Participation of E- and P- But Not L-Selectin.
- M. D. Catalina, P. Estess, and M. H. Siegelman (1999)
Blood
93, 580-589
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- L-selectin-mediated Leukocyte Adhesion In Vivo: Microvillous Distribution Determines Tethering Efficiency, But Not Rolling Velocity.
- J. V. Stein, G. Cheng, B. M. Stockton, B. P. Fors, E. C. Butcher, and U. H. von Andrian (1999)
J. Exp. Med.
189, 37-50
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- The Vascular Endothelial-Cadherin Promoter Directs Endothelial-Specific Expression in Transgenic Mice.
- S. Gory, M. Vernet, M. Laurent, E. Dejana, J. Dalmon, and P. Huber (1999)
Blood
93, 184-192
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- Two Candidate Downstream Target Genes for E2A-HLF.
- H. Kurosawa, K. Goi, T. Inukai, T. Inaba, K.-S. Chang, T. Shinjyo, K. M. Rakestraw, C. W. Naeve, and A. T. Look (1999)
Blood
93, 321-332
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- Lactosylceramide Mediates Tumor Necrosis Factor-alpha -induced Intercellular Adhesion Molecule-1 (ICAM-1) Expression and the Adhesion of Neutrophil in Human Umbilical Vein Endothelial Cells.
- A. K. Bhunia, T. Arai, G. Bulkley, and S. Chatterjee (1998)
J. Biol. Chem.
273, 34349-34357
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- Role of P-Selectin Cytoplasmic Domain in Granular Targeting In Vivo and in Early Inflammatory Responses.
- D. W. Hartwell, T. N. Mayadas, G. Berger, P. S. Frenette, H. Rayburn, R. O. Hynes, and D. D. Wagner (1998)
J. Cell Biol.
143, 1129-1141
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- Single Glycosyltransferase, Core 2 beta 1right-arrow6-N-acetylglucosaminyltransferase, Regulates Cell Surface Sialyl-Lex Expression Level in Human Pre-B Lymphocytic Leukemia Cell Line KM3 Treated with Phorbolester.
- M. Nakamura, T. Kudo, H. Narimatsu, Y. Furukawa, J. Kikuchi, S. Asakura, W. Yang, S. Iwase, K. Hatake, and Y. Miura (1998)
J. Biol. Chem.
273, 26779-26789
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- Expression of soluble endothelial adhesion molecules in clinical cardiopulmonary bypass.
- J. Galea, N. Rebuck, A. Finn, A. Manche, and N. Moat (1998)
Perfusion
13, 314-321
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