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Science 3 March 1989:
Vol. 243. no. 4895, pp. 1160 - 1165
DOI: 10.1126/science.2466335

Articles

Science, Vol 243, Issue 4895, 1160-1165
Copyright © 1989 by American Association for the Advancement of Science


articles

Endothelial leukocyte adhesion molecule 1: an inducible receptor for neutrophils related to complement regulatory proteins and lectins

MP Bevilacqua, S Stengelin, MA Gimbrone Jr, and B Seed

Department of Pathology, Brigham and Women's Hospital, Boston, MA 02115.

Focal adhesion of leukocytes to the blood vessel lining is a key step in inflammation and certain vascular disease processes. Endothelial leukocyte adhesion molecule-1 (ELAM-1), a cell surface glycoprotein expressed by cytokine-activated endothelium, mediates the adhesion of blood neutrophils. A full-length complementary DNA (cDNA) for ELAM-1 has now been isolated by transient expression in COS cells. Cells transfected with the ELAM-1 clone express a surface structure recognized by two ELAM-1 specific monoclonal antibodies (H4/18 and H18/7) and support the adhesion of isolated human neutrophils and the promyelocytic cell line HL-60. Expression of ELAM-1 transcripts in cultured human endothelial cells is induced by cytokines, reaching a maximum at 2 to 4 hours and decaying by 24 hours; cell surface expression of ELAM-1 protein parallels that of the mRNA. The primary sequence of ELAM-1 predicts an amino-terminal lectin-like domain, an EGF domain, and six tandem repetitive motifs (about 60 amino acids each) related to those found in complement regulatory proteins. A similar domain structure is also found in the MEL-14 lymphocyte cell surface homing receptor, and in granule-membrane protein 140, a membrane glycoprotein of platelet and endothelial secretory granules that can be rapidly mobilized (less than 5 minutes) to the cell surface by thrombin and other stimuli. Thus, ELAM-1 may be a member of a nascent gene family of cell surface molecules involved in the regulation of inflammatory and immunological events at the interface of vessel wall and blood.


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Adhesion Molecules in Cerebrovascular Diseases : Evidence for an Inflammatory Endothelial Activation in Cerebral Large- and Small-Vessel Disease.
K. Fassbender, T. Bertsch, O. Mielke, F. Muhlhauser, and M. Hennerici (1999)
Stroke 30, 1647-1650
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Loss of Endothelial Surface Expression of E-Selectin in a Patient With Recurrent Infections.
H. M. DeLisser, M. Christofidou-Solomidou, J. Sun, M. T. Nakada, and K. E. Sullivan (1999)
Blood 94, 884-894
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Chemokines in Lung Injury: Thomas A. Neff Lecture.
R. M. Strieter, S. L. Kunkel, M. P. Keane, and T. J. Standiford (1999)
Chest 116 , 103S-110S
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Noninflammatory Expression of E-Selectin Is Regulated by Cell Growth.
J. Luo, G. Paranya, and J. Bischoff (1999)
Blood 93, 3785-3791
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Overexpression of A1, an NF-kappa B-Inducible Anti-Apoptotic Bcl Gene, Inhibits Endothelial Cell Activation.
D. M. Stroka, A. Z. Badrichani, F. H. Bach, and C. Ferran (1999)
Blood 93, 3803-3810
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Endothelial Cells Undergoing Apoptosis Become Proadhesive for Nonactivated Platelets.
T. Bombeli, B. R. Schwartz, and J. M. Harlan (1999)
Blood 93, 3831-3838
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Pediatric AIDS-Associated Lymphocytic Interstitial Pneumonia and Pulmonary Arterio-Occlusive Disease : Role of VCAM-1/VLA-4 Adhesion Pathway and Human Herpesviruses.
S. J. Brodie, C. de la Rosa, J. G. Howe, J. Crouch, W. D. Travis, and K. Diem (1999)
Am. J. Pathol. 154, 1453-1464
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Synergistic Effects of Interleukin-4 or Interleukin-13 and Tumor Necrosis Factor-alpha on Eosinophil Activation In Vitro.
W. Luttmann, T. Matthiesen, H. Matthys, and J. C. Virchow Jr. (1999)
Am. J. Respir. Cell Mol. Biol. 20, 474-480
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Activated T Cells Acquire Endothelial Cell Surface Determinants During Transendothelial Migration.
R. I. Brezinschek, N. Oppenheimer-Marks, and P. E. Lipsky (1999)
J. Immunol. 162, 1677-1684
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Endothelial Cells Express a Novel, Tumor Necrosis Factor-alpha -regulated Variant of HOXA9.
C. V. Patel, R. Sharangpani, S. Bandyopadhyay, and P. E. DiCorleto (1999)
J. Biol. Chem. 274, 1415-1422
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Selective Requirements for Leukocyte Adhesion Molecules in Models of Acute and Chronic Cutaneous Inflammation: Participation of E- and P- But Not L-Selectin.
M. D. Catalina, P. Estess, and M. H. Siegelman (1999)
Blood 93, 580-589
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L-selectin-mediated Leukocyte Adhesion In Vivo: Microvillous Distribution Determines Tethering Efficiency, But Not Rolling Velocity.
J. V. Stein, G. Cheng, B. M. Stockton, B. P. Fors, E. C. Butcher, and U. H. von Andrian (1999)
J. Exp. Med. 189, 37-50
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The Vascular Endothelial-Cadherin Promoter Directs Endothelial-Specific Expression in Transgenic Mice.
S. Gory, M. Vernet, M. Laurent, E. Dejana, J. Dalmon, and P. Huber (1999)
Blood 93, 184-192
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Two Candidate Downstream Target Genes for E2A-HLF.
H. Kurosawa, K. Goi, T. Inukai, T. Inaba, K.-S. Chang, T. Shinjyo, K. M. Rakestraw, C. W. Naeve, and A. T. Look (1999)
Blood 93, 321-332
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Lactosylceramide Mediates Tumor Necrosis Factor-alpha -induced Intercellular Adhesion Molecule-1 (ICAM-1) Expression and the Adhesion of Neutrophil in Human Umbilical Vein Endothelial Cells.
A. K. Bhunia, T. Arai, G. Bulkley, and S. Chatterjee (1998)
J. Biol. Chem. 273, 34349-34357
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Role of P-Selectin Cytoplasmic Domain in Granular Targeting In Vivo and in Early Inflammatory Responses.
D. W. Hartwell, T. N. Mayadas, G. Berger, P. S. Frenette, H. Rayburn, R. O. Hynes, and D. D. Wagner (1998)
J. Cell Biol. 143, 1129-1141
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Single Glycosyltransferase, Core 2 beta 1right-arrow6-N-acetylglucosaminyltransferase, Regulates Cell Surface Sialyl-Lex Expression Level in Human Pre-B Lymphocytic Leukemia Cell Line KM3 Treated with Phorbolester.
M. Nakamura, T. Kudo, H. Narimatsu, Y. Furukawa, J. Kikuchi, S. Asakura, W. Yang, S. Iwase, K. Hatake, and Y. Miura (1998)
J. Biol. Chem. 273, 26779-26789
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Expression of soluble endothelial adhesion molecules in clinical cardiopulmonary bypass.
J. Galea, N. Rebuck, A. Finn, A. Manche, and N. Moat (1998)
Perfusion 13, 314-321
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