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Science 24 February 1989:
Vol. 243. no. 4894, pp. 1074 - 1076
DOI: 10.1126/science.2466334
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Articles

Science, Vol 243, Issue 4894, 1074-1076
Copyright © 1989 by American Association for the Advancement of Science

articles

Structure and function of human amphiregulin: a member of the epidermal growth factor family

M Shoyab, GD Plowman, VL McDonald, JG Bradley, and GJ Todaro

Oncogen, Seattle, WA 98121.

The complete amino acid sequence of amphiregulin, a bifunctional cell growth modulator, was determined. The truncated form contains 78 amino acids, whereas a larger form of amphiregulin contains six additional amino acids at the amino-terminal end. The amino-terminal half of amphiregulin is extremely hydrophilic and contains unusually high numbers of lysine, arginine, and asparagine residues. The carboxyl-terminal half of amphiregulin (residues 46 to 84) exhibits striking homology to the epidermal growth factor (EGF) family of proteins. Amphiregulin binds to the EGF receptor but not as well as EGF does. Amphiregulin fully supplants the requirement for EGF or transforming growth factor-alpha in murine keratinocyte growth, but it is a much weaker growth stimulator in other cell systems.


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L.-M. Wang, A. Kuo, M. Alimandi, M. C. Veri, C.-C. Lee, V. Kapoor, N. Ellmore, X.-H. Chen, and J. H. Pierce (1998)
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Increased expression of transforming growth factor alpha  precursors in acute experimental colitis in rats.
P Hoffmann, J M Zeeh, J Lakshmanan, V S Wu, F Procaccino, M Reinshagen, J A McRoberts, and V E Eysselein (1997)
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A differential display strategy identifies Cryptic, a novel EGF-related gene expressed in the axial and lateral mesoderm during mouse gastrulation.
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Development 124, 429-442
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Amino-terminal Processing of Cell Surface Heparin-binding Epidermal Growth Factor-like Growth Factor Up-regulates Its Juxtacrine but Not Its Paracrine Growth Factor Activity.
T. Nakagawa, S. Higashiyama, T. Mitamura, E. Mekada, and N. Taniguchi (1996)
J. Biol. Chem. 271, 30858-30863
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Characterization of the Mouse Epidermal Growth Factor Promoter and 5'-Flanking Region. ROLE FOR AN ATYPICAL TATA SEQUENCE.
S. E. Fenton, N. S. Groce, and D. C. Lee (1996)
J. Biol. Chem. 271, 30870-30878
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Cysteines 153 and 154 of Transmembrane Transforming Growth Factor-alpha Are Palmitoylated and Mediate Cytoplasmic Protein Association.
L. Shum, C. W. Turck, and R. Derynck (1996)
J. Biol. Chem. 271, 28502-28508
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The Epidermal Growth Factor Receptor Couples Transforming Growth Factor-alpha , Heparin-binding Epidermal Growth Factor-like Factor, and Amphiregulin to Neu, ErbB-3, and ErbB-4.
D. J. Riese II, E. D. Kim, K. Elenius, S. Buckley, M. Klagsbrun, G. D. Plowman, and D. F. Stern (1996)
J. Biol. Chem. 271, 20047-20052
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COOH-terminal Extended Recombinant Amphiregulin with Bioactivity Comparable with Naturally Derived Growth Factor.
S. A. Thompson, A. Harris, D. Hoang, M. Ferrer, and G. R. Johnson (1996)
J. Biol. Chem. 271, 17927-17931
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Insulin Regulates Heregulin Binding and ErbB3 Expression in Rat Hepatocytes.
R. S. Carver, M. X. Sliwkowski, S. Sitaric, and W. E. Russell (1996)
J. Biol. Chem. 271, 13491-13496
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Epidermal Growth Factor-related Peptides Activate Distinct Subsets of ErbB Receptors and Differ in Their Biological Activities.
R. R. Beerli and N. E. Hynes (1996)
J. Biol. Chem. 271, 6071-6076
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Amphiregulin in lung branching morphogenesis: interaction with heparan sulfate proteoglycan modulates cell proliferation.
L Schuger, G. Johnson, K Gilbride, G. Plowman, and R Mandel (1996)
Development 122, 1759-1767
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HER4 Receptor Activation and Phosphorylation of Shc Proteins by Recombinant Heregulin-Fc Fusion Proteins.
J.-M. Culouscou, G. W. Carlton, and A. Aruffo (1995)
J. Biol. Chem. 270, 12857-12863
   Abstract »    Full Text »    PDF »
Epiregulin.
H. Toyoda, T. Komurasaki, D. Uchida, Y. Takayama, T. Isobe, T. Okuyama, and K. Hanada (1995)
J. Biol. Chem. 270, 7495-7500
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TGF alpha can act as a chemoattractant to perioptic mesenchymal cells in developing mouse eyes.
L. Reneker, D. Silversides, K Patel, and P. Overbeek (1995)
Development 121, 1669-1680
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The mouse waved-2 phenotype results from a point mutation in the EGF receptor tyrosine kinase..
N C Luetteke, H K Phillips, T H Qiu, N G Copeland, H S Earp, N A Jenkins, and D C Lee (1994)
Genes & Dev. 8, 399-413
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The heparin-binding domain of heparin-binding EGF-like growth factor can target Pseudomonas exotoxin to kill cells exclusively through heparan sulfate proteoglycans.
E. Mesri, M Ono, R. Kreitman, M Klagsbrun, and I Pastan (1994)
J. Cell Sci. 107, 2599-2608
   Abstract »    PDF »
The murine cripto gene: expression during mesoderm induction and early heart morphogenesis.
R Dono, L Scalera, F Pacifico, D Acampora, M. Persico, and A Simeone (1993)
Development 118, 1157-1168
   Abstract »    PDF »
A genetic tool used to identify thioredoxin as a mediator of a growth inhibitory signal.
L. Deiss and A Kimchi (1991)
Science 252, 117-120
   Abstract »    PDF »
A heparin-binding growth factor secreted by macrophage-like cells that is related to EGF.
S Higashiyama, J. Abraham, J Miller, J. Fiddes, and M Klagsbrun (1991)
Science 251, 936-939
   Abstract »    PDF »
Cyclosporine Inhibits Basic Fibroblast Growth Factor-Driven Proliferation of Human Endothelial Cells and Keratinocytes.
R. J. Sharpe, K. A. Arndt, S. I. Bauer, and T. E. Maione (1989)
Arch Dermatol 125, 1359-1362
   Abstract »    PDF »
Cloning and Biological Activity of Epigen, a Novel Member of the Epidermal Growth Factor Superfamily.
L. Strachan, J. G. Murison, R. L. Prestidge, M. A. Sleeman, J. D. Watson, and K. D. Kumble (2001)
J. Biol. Chem. 276, 18265-18271
   Abstract »    Full Text »    PDF »
Novel Betacellulin Derivatives. SEPARATION OF THE DIFFERENTIATION ACTIVITY FROM THE MITOGENIC ACTIVITY.
T. Itoh, M. Kondo, Y. Tanaka, M. Kobayashi, R. Sasada, K. Igarashi, M. Suenaga, N. Koyama, O. Nishimura, and M. Fujino (2001)
J. Biol. Chem. 276, 40698-40703
   Abstract »    Full Text »    PDF »


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