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Science 3 February 1989:
Vol. 243. no. 4891, pp. 652 - 654
DOI: 10.1126/science.2492677
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Articles

Science, Vol 243, Issue 4891, 652-654
Copyright © 1989 by American Association for the Advancement of Science

articles

Reversible cleavage and ligation of hepatitis delta virus RNA

HN Wu and MM Lai

Institute of Molecular Biology, Academia Sinica, Taipei, Taiwan.

A 148-nucleotide subfragment of hepatitis delta virus RNA was shown to undergo cleavage and ligation reversibly. The direction of the reaction is determined by the presence or absence of Mg2+ ions, with the presence of Mg2+ favoring the cleavage reaction. Ligation requires specific conformation of the RNA molecules involved and occurs only between two cleaved RNA fragments that are still held together by hydrogen bonds. The ligation reaction occurs rapidly on removal of Mg2+ by EDTA. This represents a new class of RNA enzymes.


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Erratum.
(1989)
Science 243, 1420
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