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Science 27 January 1989: Vol. 243. no. 4890, pp. 538 - 542 DOI: 10.1126/science.2911757
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Articles
Science, Vol 243, Issue 4890, 538-542
Copyright © 1989 by American Association for the Advancement of Science
Evidence that the leucine zipper is a coiled coil
EK O'Shea,
R Rutkowski,
and
PS Kim
Whitehead Institute for Biomedical Research, Cambridge, MA 02142.
Recently, a hypothetical structure called a leucine zipper was proposed that defines a new class of DNA binding proteins. The common feature of these proteins is a region spanning approximately 30 amino acids that contains a periodic repeat of leucines every seven residues. A peptide corresponding to the leucine zipper region of the yeast transcriptional activator GCN4 was synthesized and characterized. This peptide associates in the micromolar concentration range to form a very stable dimer of alpha helices with a parallel orientation. Although some features of the leucine zipper model are supported by our experimental data, the peptide has the characteristics of a coiled coil.
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Genes & Dev.
4, 2025-2035
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- Identification of HTLV-I tax trans-activator mutants exhibiting novel transcriptional phenotypes..
- M R Smith and W C Greene (1990)
Genes & Dev.
4, 1875-1885
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- De novo design, expression, and characterization of Felix: a four-helix bundle protein of native-like sequence.
- M. Hecht, J. Richardson, D. Richardson, and R. Ogden (1990)
Science
249, 884-891
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- Sequence-specific DNA binding by a short peptide dimer.
- R. Talanian, C. McKnight, and P. Kim (1990)
Science
249, 769-771
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- Evidence of changes in protease sensitivity and subunit exchange rate on DNA binding by C/EBP.
- J. Shuman, C. Vinson, and S. McKnight (1990)
Science
249, 771-774
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- Design of DNA-binding peptides based on the leucine zipper motif.
- K. O'Neil, R. Hoess, and W. DeGrado (1990)
Science
249, 774-778
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- Ig/EBP-1: a ubiquitously expressed immunoglobulin enhancer binding protein that is similar to C/EBP and heterodimerizes with C/EBP..
- C Roman, J S Platero, J Shuman, and K Calame (1990)
Genes & Dev.
4, 1404-1415
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- Structural motif of the GCN4 DNA binding domain characterized by affinity cleaving.
- M. Oakley and P. Dervan (1990)
Science
248, 847-850
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- TFE3: a helix-loop-helix protein that activates transcription through the immunoglobulin enhancer muE3 motif..
- H Beckmann, L K Su, and T Kadesch (1990)
Genes & Dev.
4, 167-179
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- Different requirements for formation of Jun: Jun and Jun: Fos complexes..
- T Smeal, P Angel, J Meek, and M Karin (1989)
Genes & Dev.
3, 2091-2100
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