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Science 27 January 1989:
Vol. 243. no. 4890, pp. 522 - 526
DOI: 10.1126/science.2536192
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Articles

Science, Vol 243, Issue 4890, 522-526
Copyright © 1989 by American Association for the Advancement of Science

articles

The effect of GTPase activating protein upon ras is inhibited by mitogenically responsive lipids

MH Tsai, CL Yu, FS Wei, and DW Stacey

Department of Molecular Biology, Cleveland Clinic Foundation, OH 44106.

Bacterially synthesized c-Ha-ras protein (Ras) was incubated with guanosine triphosphatase (GTPase) activating (GA) protein in the presence of various phospholipids. The stimulation of Ras GTPase activity by GA protein was inhibited in some cases. Among the lipids most active in blocking GA protein activity were lipids that show altered metabolism during mitogenic stimulation. These included phosphatidic acid (containing arachidonic acid), phosphatidylinositol phosphates, and arachidonic acid. Other lipids, including phosphatidic acid with long, saturated side chains, diacylglycerols, and many other common phospholipids, were unable to alter GA protein activity. The interaction of lipids with GA protein might be important in the regulation of Ras activity during mitogenic stimulation.


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