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Science 23 December 1988:
Vol. 242. no. 4886, pp. 1706 - 1709
DOI: 10.1126/science.2904703
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Articles

Science, Vol 242, Issue 4886, 1706-1709
Copyright © 1988 by American Association for the Advancement of Science

articles

Engraftment of immune-deficient mice with human hematopoietic stem cells

S Kamel-Reid and JE Dick

Department of Genetics, Hospital for Sick Children, Toronto, Ontario.

A system in which immune-deficient mice are repopulated with cells from the human myeloid lineage, and that provides an in vivo stem cell assay for human hematopoietic cells is described. Generation of the chimeric human/immune-deficient (HID) mice was dependent on the use of immune-deficient bg/nu/xid mice. Infusion of these mice with human bone marrow gave rise to increases in human macrophage progenitors during more than 5 weeks of in vivo growth, indicating the seeding, proliferation, and differentiation of human stem cells. The human identity of the progenitors was confirmed by sequence analysis and their dependence on human growth factors. The creation of HID mice lays the foundation for establishing animal models for a wide variety of human hemopathies, from leukemia to infectious disease.


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