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Science 18 November 1988:
Vol. 242. no. 4881, pp. 1056 - 1058
DOI: 10.1126/science.2461587
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Articles

Science, Vol 242, Issue 4881, 1056-1058
Copyright © 1988 by American Association for the Advancement of Science

articles

Activation of developmentally mutated human globin genes by cell fusion

T Papayannopoulou, T Enver, S Takegawa, NP Anagnou, and G Stamatoyannopoulos

Division of Hematology, University of Washington, Seattle 98195.

Human fetal globin genes are not expressed in hybrid cells produced by the fusion of normal human lymphocytes with mouse erythroleukemia cells. In contrast, when lymphocytes from persons with globin gene developmental mutations (hereditary persistence of fetal hemoglobin) are used for these fusions, fetal globin is expressed in the hybrid cells. Thus, mutations of developmental origin can be reconstituted in vitro by fusing mutant lymphoid cells with differentiated cell lines of the proper lineage. This system can readily be used for analyses, such as globin gene methylation, that normally require large numbers of pure nucleated erythroid cells, which are difficult to obtain.


THIS ARTICLE HAS BEEN CITED BY OTHER ARTICLES:
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Comparison of Expression of Human Globin Genes Transferred Into Mouse Erythroleukemia Cells and in Transgenic Mice.
E. Skarpidi, G. Vassilopoulos, G. Stamatoyannopoulos, and Q. Li (1998)
Blood 92, 3416-3421
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A deletion of the human beta-globin locus activation region causes a major alteration in chromatin structure and replication across the entire beta-globin locus..
W C Forrester, E Epner, M C Driscoll, T Enver, M Brice, T Papayannopoulou, and M Groudine (1990)
Genes & Dev. 4, 1637-1649
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Science. ISSN 0036-8075 (print), 1095-9203 (online)