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Science 18 November 1988:
Vol. 242. no. 4881, pp. 1028 - 1035
DOI: 10.1126/science.2973660
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Articles

Science, Vol 242, Issue 4881, 1028-1035
Copyright © 1988 by American Association for the Advancement of Science

articles

An early hierarchic role of U1 small nuclear ribonucleoprotein in spliceosome assembly

SW Ruby and J Abelson

Division of Biology, California Institute of Technology, Pasadena 91125.

Splicing of nuclear precursor messenger RNA (pre-mRNA) occurs on a large ribonucleoprotein complex, the spliceosome. Several small nuclear ribonucleoproteins (snRNP's) are subunits of this complex that assembles on the pre-mRNA. Although the U1 snRNP is known to recognize the 5' splice site, its roles in spliceosome formation and splice site alignment have been unclear. A new affinity purification method for the spliceosome is described which has provided insight into the very early stages of spliceosome formation in a yeast in vitro splicing system. Surprisingly, the U1 snRNP initially recognizes sequences at or near both splice junctions in the intron. This interaction must occur before the other snRNP's (U2, U4, U5, and U6) can join the complex. The results suggest that interaction of the two splice site regions occurs at an early stage of spliceosome formation and is probably mediated by U1 snRNP and perhaps other factors.


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Regulation of splicing at an intermediate step in the formation of the spliceosome..
J Vilardell and J R Warner (1994)
Genes & Dev. 8, 211-220
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Four yeast spliceosomal proteins (PRP5, PRP9, PRP11, and PRP21) interact to promote U2 snRNP binding to pre-mRNA..
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A functional association between the 5' and 3' splice site is established in the earliest prespliceosome complex (E) in mammals..
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Genes & Dev. 7, 1008-1020
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Association with terminal exons in pre-mRNAs: a new role for the U1 snRNP?.
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Efficient association of U2 snRNPs with pre-mRNA requires an essential U2 RNA structural element..
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In vivo UV cross-linking of U snRNAs that participate in trypanosome trans-splicing..
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U1 snRNP can influence 3'-splice site selection as well as 5'-splice site selection..
V Goguel, X L Liao, B C Rymond, and M Rosbash (1991)
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Immunoaffinity purification of a [U4/U6.U5] tri-snRNP from human cells..
S E Behrens and R Luhrmann (1991)
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Messenger RNA splicing in yeast: clues to why the spliceosome is a ribonucleoprotein.
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A cold-sensitive mRNA splicing mutant is a member of the RNA helicase gene family..
E J Strauss and C Guthrie (1991)
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Alpha-tropomyosin mutually exclusive exon selection: competition between branchpoint/polypyrimidine tracts determines default exon choice..
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D L Black (1991)
Genes & Dev. 5, 389-402
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Control of alternative splicing by the differential binding of U1 small nuclear ribonucleoprotein particle.
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Lethal and temperature-sensitive mutations and their suppressors identify an essential structural element in U2 small nuclear RNA..
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Evidence for base-pairing between mammalian U2 and U6 small nuclear ribonucleoprotein particles..
T P Hausner, L M Giglio, and A M Weiner (1990)
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Two domains of yeast U6 small nuclear RNA required for both steps of nuclear precursor messenger RNA splicing.
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Universally conserved and yeast-specific U1 snRNA sequences are important but not essential for U1 snRNP function..
X L Liao, L Kretzner, B Seraphin, and M Rosbash (1990)
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A Sequential splicing mechanism promotes selection of an optimal exon by repositioning a downstream 5' splice site in preprotachykinin pre-mRNA..
F H Nasim, P A Spears, H M Hoffmann, H C Kuo, and P J Grabowski (1990)
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U1-specific protein C needed for efficient complex formation of U1 snRNP with a 5' splice site.
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Identification and purification of a 62,000-dalton protein that binds specifically to the polypyrimidine tract of introns..
M A Garcia-Blanco, S F Jamison, and P A Sharp (1989)
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U2 snRNA sequences that bind U2-specific proteins are dispensable for the function of U2 snRNP in splicing..
Z Q Pan and C Prives (1989)
Genes & Dev. 3, 1887-1898
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In vitro reconstitution of functional yeast U2 snRNPs..
D S McPheeters, P Fabrizio, and J Abelson (1989)
Genes & Dev. 3, 2124-2136
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In vitro assembly of yeast U6 snRNP: a functional assay..
P Fabrizio, D S McPheeters, and J Abelson (1989)
Genes & Dev. 3, 2137-2150
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A compensatory base change in human U2 snRNA can suppress a branch site mutation..
Y Zhuang and A M Weiner (1989)
Genes & Dev. 3, 1545-1552
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Isolation and characterization of pre-mRNA splicing mutants of Saccharomyces cerevisiae..
U Vijayraghavan, M Company, and J Abelson (1989)
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New Roles for the Snp1 and Exo84 Proteins in Yeast Pre-mRNA Splicing.
S. Awasthi, R. Palmer, M. Castro, C. D. Mobarak, and S. W. Ruby (2001)
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Science. ISSN 0036-8075 (print), 1095-9203 (online)