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Science 14 October 1988:
Vol. 242. no. 4876, pp. 275 - 278
DOI: 10.1126/science.3051382
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Articles

Science, Vol 242, Issue 4876, 275-278
Copyright © 1988 by American Association for the Advancement of Science

articles

Antibodies to Asp-Asp-Glu-Asp can inhibit transport of nuclear proteins into the nucleus

Y Yoneda, N Imamoto-Sonobe, Y Matsuoka, R Iwamoto, Y Kiho, and T Uchida

Institute for Molecular and Cellular Biology, Osaka University, Japan.

The signal sequence of simian virus 40 (SV40) large T-antigen for translocation into the nucleus is composed of positively charged amino acids Lys-Lys-Lys-Arg-Lys. Rabbit antibodies to a synthetic peptide containing the negatively charged amino acid sequence Asp-Asp-Asp-Glu-Asp were obtained. Indirect immunofluorescence of the antigens recognized by the antibody was punctate at the nuclear rim or the nuclear surface, depending on the plane of focus. The antibody blocked transport of nuclear proteins into the nucleus. The antigens recognized by the antibody were predominantly localized to the nuclear pores.


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