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Science 14 October 1988:
Vol. 242. no. 4876, pp. 261 - 263
DOI: 10.1126/science.3140378
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Articles

Science, Vol 242, Issue 4876, 261-263
Copyright © 1988 by American Association for the Advancement of Science

articles

Unusual immunoglobulin gene rearrangement leads to replacement of recombinational signal sequences

E Morzycka-Wroblewska, FE Lee, and SV Desiderio

Howard Hughes Medical Institute Laboratory of Genetics, Johns Hopkins University School of Medicine, Baltimore, MD 21205.

An unexpected immunoglobulin gene rearrangement, signal sequence replacement, was observed in which the recombinational signal sequences of a VH gene segment are fused intact to the 5' end of a DJH element. Nucleotides are not lost from the signal sequences, but they may be lost from the DJH coding sequence. Signal sequence replacement may result from the alternative resolution of an intermediate in VH-to-DJH recombination. This type of rearrangement provides a means to alter the targeting of immunoglobulin gene segments and suggests a mechanism for the occurrence of VH-JH junctions in vivo. Signal sequence replacement may represent an additional pathway for the generation of antibody diversity.


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