Note to users. If you're seeing this message, it means that your browser cannot find this page's style/presentation instructions -- or possibly that you are using a browser that does not support current Web standards. Find out more about why this message is appearing, and what you can do to make your experience of our site the best it can be.

Science 26 August 1988:
Vol. 241. no. 4869, pp. 1089 - 1092
DOI: 10.1126/science.2970670
Prev | Table of Contents | Next

Articles

Science, Vol 241, Issue 4869, 1089-1092
Copyright © 1988 by American Association for the Advancement of Science

articles

Isotypic exclusion of gamma delta T cell receptors in transgenic mice bearing a rearranged beta-chain gene

RG Fenton, P Marrack, JW Kappler, O Kanagawa, and JG Seidman

Department of Genetics, Harvard Medical School, Boston, MA 02115.

The rearrangement of T cell antigen receptor beta- and gamma-chain gene segments was studied in transgenic mice that bear a functional beta-chain gene. Virtually all CD3-positive T cells derived from transgenic mice express beta chains containing the transgene-encoded V beta 8.2 variable region on their surfaces and do not express endogenous beta-chain variable regions. Expression of endogenous V beta genes is inhibited at the level of somatic recombination during thymic ontogeny. Furthermore, rearrangements of the TCR gamma-chain genes are also markedly inhibited in these transgenic animals. Hence expression of the TCR beta transgene has led to allelic exclusion of alpha beta receptors and isotypic exclusion of gamma delta T cell receptors.


THIS ARTICLE HAS BEEN CITED BY OTHER ARTICLES:
CD8 T Cells Require Bcl-3 for Maximal Gamma Interferon Production upon Secondary Exposure to Antigen.
P. M. Chilton and T. C. Mitchell (2006)
Infect. Immun. 74, 4180-4189
   Abstract »    Full Text »    PDF »
Bax does not have to adopt its final form to drive T cell death.
Y. Zhu, X. Liu, D. Hildeman, F. W. Peyerl, J. White, E. Kushnir, J. Kappler, and P. Marrack (2006)
J. Exp. Med. 203, 1147-1152
   Abstract »    Full Text »    PDF »
Early Expression of a Functional TCR{beta} Chain Inhibits TCR{gamma} Gene Rearrangements without Altering the Frequency of TCR{gamma}{delta} Lineage Cells.
D. Gerber, L. Boucontet, and P. Pereira (2004)
J. Immunol. 173, 2516-2523
   Abstract »    Full Text »    PDF »
Constitutive association of the proapoptotic protein Bim with Bcl-2-related proteins on mitochondria in T cells.
Y. Zhu, B. J. Swanson, M. Wang, D. A. Hildeman, B. C. Schaefer, X. Liu, H. Suzuki, K. Mihara, J. Kappler, and P. Marrack (2004)
PNAS 101, 7681-7686
   Abstract »    Full Text »    PDF »
Control of Bcl-2 expression by reactive oxygen species.
D. A. Hildeman, T. Mitchell, B. Aronow, S. Wojciechowski, J. Kappler, and P. Marrack (2003)
PNAS 100, 15035-15040
   Abstract »    Full Text »    PDF »
Analysis and expression of a cloned pre-T cell receptor gene.
C Saint-Ruf, K Ungewiss, M Groettrup, L Bruno, H. Fehling, and H von Boehmer (1994)
Science 266, 1208-1212
   Abstract »    PDF »
Progression to diabetes in nonobese diabetic (NOD) mice with transgenic T cell receptors.
M. Lipes, A Rosenzweig, K. Tan, G Tanigawa, D Ladd, J. Seidman, and G. Eisenbarth (1993)
Science 259, 1165-1169
   Abstract »    PDF »


To Advertise     Find Products

Science. ISSN 0036-8075 (print), 1095-9203 (online)