Note to users. If you're seeing this message, it means that your browser cannot find this page's style/presentation instructions -- or possibly that you are using a browser that does not support current Web standards. Find out more about why this message is appearing, and what you can do to make your experience of our site the best it can be.

Site Tools

  • AAAS
  • Subscribe
  • Feedback

Site Search

Search Advanced

Science 26 August 1988:
Vol. 241. no. 4869, pp. 1064 - 1069
DOI: 10.1126/science.2457948
Prev | Table of Contents | Next

Articles

Science, Vol 241, Issue 4869, 1064-1069
Copyright © 1988 by American Association for the Advancement of Science

articles

Ordered interstrand and intrastrand DNA transfer during reverse transcription

AT Panganiban and D Fiore

McArdle Laboratory for Cancer Research, University of Wisconsin, Madison 53706.

Retroviruses contain two copies of the plus stranded viral RNA genome. As a means of determining whether both of these RNA's are used in the reverse transcription reaction, cells were infected with heterozygous virus particles that varied in nucleotide sequence at two separate locations at the RNA termini. The DNA proviruses formed from a single cycle of reverse transcription were then examined. Of the 12 proviruses that were characterized, all exhibited long terminal repeats (LTR's) that would be expected to arise only if both RNA templates were used for the generation of minus strand DNA. In contrast, only a single minus strand DNA appeared to be used as template for the plus strand DNA in the generation of fully double-stranded viral DNA. These results indicate that the first strand transfer step in reverse transcription is an intermolecular event while that of the second transfer is intramolecular. Thus, retroviruses contain two functionally active RNA's, and both may be required for the generation of a single linear DNA molecule. Formation of heterozygotes during retrovirus infection would be expected to result in the efficient generation of LTR recombinants.


THIS ARTICLE HAS BEEN CITED BY OTHER ARTICLES:
Pseudodiploid Genome Organization Aids Full-Length Human Immunodeficiency Virus Type 1 DNA Synthesis.
S. R. King, N. K. Duggal, C. B. Ndongmo, C. Pacut, and A. Telesnitsky (2008)
J. Virol. 82, 2376-2384
   Abstract »    Full Text »    PDF »
High rate of genetic recombination in murine leukemia virus: implications for influencing proviral ploidy..
J. Zhuang, S. Mukherjee, Y. Ron, and J. P. Dougherty (2006)
J. Virol. 80, 6706-6711
   Abstract »    Full Text »    PDF »
A new NMR solution structure of the SL1 HIV-1Lai loop-loop dimer.
F. Kieken, F. Paquet, F. Brule, J. Paoletti, and G. Lancelot (2006)
Nucleic Acids Res. 34, 343-352
   Abstract »    Full Text »    PDF »
Structural probing of a pathogenic tRNA dimer.
M. D. ROY, L. M. WITTENHAGEN, and S. O. KELLEY (2005)
RNA 11, 254-260
   Abstract »    Full Text »    PDF »
Nonrandom Dimerization of Murine Leukemia Virus Genomic RNAs.
J. A. Flynn, W. An, S. R. King, and A. Telesnitsky (2004)
J. Virol. 78, 12129-12139
   Abstract »    Full Text »    PDF »
Template Dimerization Promotes an Acceptor Invasion-Induced Transfer Mechanism during Human Immunodeficiency Virus Type 1 Minus-Strand Synthesis.
M. Balakrishnan, B. P. Roques, P. J. Fay, and R. A. Bambara (2003)
J. Virol. 77, 4710-4721
   Abstract »    Full Text »    PDF »
Intramolecular Recombinations of Moloney Murine Leukemia Virus Occur during Minus-Strand DNA Synthesis.
T. Li and J. Zhang (2002)
J. Virol. 76, 9614-9623
   Abstract »    Full Text »    PDF »
Dissociation of Genome Dimerization from Packaging Functions and Virion Maturation of Human Immunodeficiency Virus Type 1.
J.-i. Sakuragi, A. Iwamoto, and T. Shioda (2002)
J. Virol. 76, 959-967
   Abstract »    Full Text »    PDF »
Effects of Limiting Homology at the Site of Intermolecular Recombinogenic Template Switching during Moloney Murine Leukemia Virus Replication.
J. K. Pfeiffer and A. Telesnitsky (2001)
J. Virol. 75, 11263-11274
   Abstract »    Full Text »
Evidence for the Packaging of Multiple Copies of Tf1 mRNA into Particles and the trans Priming of Reverse Transcription.
A. L. Haag, J.-H. Lin, and H. L. Levin (2000)
J. Virol. 74, 7164-7170
   Abstract »    Full Text »
Role of Post-transcriptional Modifications of Primer tRNALys,3 in the Fidelity and Efficacy of Plus Strand DNA Transfer during HIV-1 Reverse Transcription.
S. Auxilien, G. Keith, S. F. J. Le Grice, and J.-L. Darlix (1999)
J. Biol. Chem. 274, 4412-4420
   Abstract »    Full Text »    PDF »
Genetic Analysis of the Rat Leukemia Virus: Influence of Viral Sequences in Transduction of the c-ras Proto-Oncogene and Expression of Its Transforming Activity.
S.-Y. Lee, T. M. Howard, and S. Rasheed (1998)
J. Virol. 72, 9906-9917
   Abstract »    Full Text »    PDF »
The Nature of Human Immunodeficiency Virus Type 1 Strand Transfers.
H. Yu, A. E. Jetzt, Y. Ron, B. D. Preston, and J. P. Dougherty (1998)
J. Biol. Chem. 273, 28384-28391
   Abstract »    Full Text »    PDF »
Extended Minus-Strand DNA as Template for R-U5-Mediated Second-Strand Transfer in Recombinational Rescue of Primer Binding Site-Modified Retroviral Vectors.
J. G. Mikkelsen, A. H. Lund, K. Dybkar, M. Duch, and F. S. Pedersen (1998)
J. Virol. 72, 2519-2525
   Abstract »    Full Text »    PDF »
Correlated Template-Switching Events during Minus-Strand DNA Synthesis: a Mechanism for High Negative Interference during Retroviral Recombination.
J. A. Anderson, R. J. Teufel II, P. D. Yin, and W.-S. Hu (1998)
J. Virol. 72, 1186-1194
   Abstract »    Full Text »    PDF »
Evidence for a Unique Mechanism of Strand Transfer from the Transactivation Response Region of HIV-1.
J. K. Kim, C. Palaniappan, W. Wu, P. J. Fay, and R. A. Bambara (1997)
J. Biol. Chem. 272, 16769-16777
   Abstract »    Full Text »    PDF »
Mechanisms of Inhibition of in Vitro Dimerization of HIV Type I RNA by Sense and Antisense Oligonucleotides.
E. Skripkin, J.-C. Paillart, R. Marquet, M. Blumenfeld, B. Ehresmann, and C. Ehresmann (1996)
J. Biol. Chem. 271, 28812-28817
   Abstract »    Full Text »    PDF »
Dimerization of HIV-1[IMAGE] RNA at Low Ionic Strength.
D. Muriaux, P.-M. Girard, Bénéd. Bonnet-Mathonière, and J. Paoletti (1995)
J. Biol. Chem. 270, 8209-8216
   Abstract »    Full Text »    PDF »
Tethering ribozymes to a retroviral packaging signal for destruction of viral RNA.
B. Sullenger and T. Cech (1993)
Science 262, 1566-1569
   Abstract »    PDF »
Mechanism of DNA strand transfer reactions catalyzed by HIV-1 reverse transcriptase.
J. Peliska and S. Benkovic (1992)
Science 258, 1112-1118
   Abstract »    PDF »
Quiescent T lymphocytes as an inducible virus reservoir in HIV-1 infection.
M. Bukrinsky, T. Stanwick, M. Dempsey, and M Stevenson (1991)
Science 254, 423-427
   Abstract »    PDF »
Crystal structure of the ribonuclease H domain of HIV-1 reverse transcriptase.
J. Davies 2nd, Z Hostomska, Z Hostomsky, Jordan SR, and D. Matthews (1991)
Science 252, 88-95
   Abstract »    PDF »
Retroviral recombination and reverse transcription.
W. Hu and H. Temin (1990)
Science 250, 1227-1233
   Abstract »    PDF »
A specific terminal structure is required for Ty1 transposition..
D J Eichinger and J D Boeke (1990)
Genes & Dev. 4, 324-330
   Abstract »    PDF »
The Kissing Hairpin Sequence Promotes Recombination within the HIV-I 5' Leader Region.
M. Balakrishnan, P. J. Fay, and R. A. Bambara (2001)
J. Biol. Chem. 276, 36482-36492
   Abstract »    Full Text »    PDF »


ADVERTISEMENT
Click Me!

ADVERTISEMENT

To Advertise     Find Products

Science. ISSN 0036-8075 (print), 1095-9203 (online)