Note to users. If you're seeing this message, it means that your browser cannot find this page's style/presentation instructions -- or possibly that you are using a browser that does not support current Web standards. Find out more about why this message is appearing, and what you can do to make your experience of our site the best it can be.

Science 12 August 1988:
Vol. 241. no. 4867, pp. 837 - 839
DOI: 10.1126/science.3406741
Prev | Table of Contents | Next

Articles

Science, Vol 241, Issue 4867, 837-839
Copyright © 1988 by American Association for the Advancement of Science

articles

Legless, a novel mutation found in PHT1-1 transgenic mice

JD McNeish, WJ Scott Jr, and SS Potter

Division of Basic Science Research, Children's Hospital Research Foundation, Cincinnati, OH.

In this report it is shown that the PHT1-1 line of transgenic mice exhibited a pattern of developmental abnormalities when the mice were homozygous for the transgene insertion. Hindlimbs were uniformly truncated at the distal end of the femur, resulting in a "legless" appearance. Forelimbs lacked anterior structures including digits and the radius. The brains had many defects, particularly in the anterior structures of the cerebrum, including the olfactory lobes. Craniofacial malformations in the form of facial clefts also commonly occurred. Furthermore, heterozygotes of this line, with only one copy of the DNA insertion, and other transgenic lines carrying the same DNA construct appeared normal, suggesting that in the PHT1-1 line a gene significant in mammalian development has been disrupted.


THIS ARTICLE HAS BEEN CITED BY OTHER ARTICLES:
The cleft lip and palate defects in Dancer mutant mice result from gain of function of the Tbx10 gene.
J. O. Bush, Y. Lan, and R. Jiang (2004)
PNAS 101, 7022-7027
   Abstract »    Full Text »    PDF »
From the Cover: Sp8 is crucial for limb outgrowth and neuropore closure.
S. M. Bell, C. M. Schreiner, R. R. Waclaw, K. Campbell, S. S. Potter, and W. J. Scott (2003)
PNAS 100, 12195-12200
   Abstract »    Full Text »    PDF »
GENETICALLY ALTERED MOUSE MODELS: THE GOOD, THE BAD, AND THE UGLY.
T. Thyagarajan, S. Totey, M. J. S. Danton, and A. B. Kulkarni (2003)
Critical Reviews in Oral Biology & Medicine 14, 154-174
   Abstract »    Full Text »    PDF »
Use of Genetically Engineered Mice in Drug Discovery and Development: Wielding Occam's Razor to Prune the Product Portfolio.
B. Bolon and E. Galbreath (2002)
International Journal of Toxicology 21, 55-64
   Abstract »    PDF »
Programmed cell death is affected in the novel mouse mutant Fused toes (Ft).
F. van der Hoeven, T. Schimmang, A. Volkmann, M. G. Mattei, B. Kyewski, and U. Ruther (1994)
Development 120, 2601-2607
   Abstract »    PDF »
The chicken limb deformity gene encodes nuclear proteins expressed in specific cell types during morphogenesis..
A Trumpp, P A Blundell, J L de la Pompa, and R Zeller (1992)
Genes & Dev. 6, 14-28
   Abstract »    PDF »
legless insertional mutation: morphological, molecular, and genetic characterization..
G Singh, D M Supp, C Schreiner, J McNeish, H J Merker, N G Copeland, N A Jenkins, S S Potter, and W Scott (1991)
Genes & Dev. 5, 2245-2255
   Abstract »    PDF »
The limb deformity gene is required for apical ectodermal ridge differentiation and anteroposterior limb pattern formation..
R Zeller, L Jackson-Grusby, and P Leder (1989)
Genes & Dev. 3, 1481-1492
   Abstract »    PDF »


To Advertise     Find Products

Science. ISSN 0036-8075 (print), 1095-9203 (online)