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Science 29 July 1988:
Vol. 241. no. 4865, pp. 577 - 580
DOI: 10.1126/science.3399892
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Articles

Science, Vol 241, Issue 4865, 577-580
Copyright © 1988 by American Association for the Advancement of Science

articles

Cloning of a lymphoid-specific cDNA encoding a protein binding the regulatory octamer DNA motif

LM Staudt, RG Clerc, H Singh, JH LeBowitz, PA Sharp, and D Baltimore

Whitehead Institute for Biomedical Research, Cambridge, MA 02142.

An octamer DNA sequence plays a critical role in directing transcription of immunoglobulin genes in B lymphocytes. A new technique of direct binding of radioactive DNA was used to screen a complementary DNA expression library from the BJAB cell line in lambda gt11 phage to derive molecular cDNA clones representing a putative B lymphocyte-specific octamer binding protein. The plaques were screened with DNA containing four copies of the octamer sequence and positive phage recombinants were identified. The fusion protein produced on inducing a lysogen of one phage bound to a monomeric octamer probe. The cDNA insert from this phage hybridized to messenger RNA found in B lymphocytes, but not in most other cells. Thus, this cDNA derives from a gene (oct-2) that specifies an octamer binding protein expressed preferentially in B lymphocytes, proving that, for at least one gene, a cell-specific transcription factor exists and its amount is controlled through messenger RNA availability.


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