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Science 22 July 1988:
Vol. 241. no. 4864, pp. 456 - 459
DOI: 10.1126/science.3293213
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Articles
Science, Vol 241, Issue 4864, 456-459
Copyright © 1988 by American Association for the Advancement of Science
Site-specific oligonucleotide binding represses transcription of the human c-myc gene in vitro
M Cooney,
G Czernuszewicz,
EH Postel,
SJ Flint,
and
ME Hogan
Department of Molecular Biology, Princeton University, NJ 08544.
A 27-base-long DNA oligonucleotide was designed that binds to duplex DNA at a single site within the 5' end of the human c-myc gene, 115 base pairs upstream from the transcription origin P1. On the basis of the physical properties of its bound complex, it was concluded that the oligonucleotide forms a colinear triplex with the duplex binding site. By means of an in vitro assay system, it was possible to show a correlation between triplex formation at -115 base pairs and repression of c-myc transcription. The possibility is discussed that triplex formation (site-specific RNA binding to a DNA duplex) could serve as the basis for an alternative program of gene control in vivo.
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