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Science 1 July 1988:
Vol. 241. no. 4861, pp. 53 - 57
DOI: 10.1126/science.3388019
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Articles

Science, Vol 241, Issue 4861, 53-57
Copyright © 1988 by American Association for the Advancement of Science

articles

Combinatorial cassette mutagenesis as a probe of the informational content of protein sequences

JF Reidhaar-Olson and RT Sauer

Department of Biology, Massachusetts Institute of Technology, Cambridge 02139.

A method of combinatorial cassette mutagenesis was designed to readily determine the informational content of individual residues in protein sequences. The technique consists of simultaneously randomizing two or three positions by oligonucleotide cassette mutagenesis, selecting for functional protein, and then sequencing to determine the spectrum of allowable substitutions at each position. Repeated application of this method to the dimer interface of the DNA-binding domain of lambda repressor reveals that the number and type of substitutions allowed at each position are extremely variable. At some positions only one or two residues are functionally acceptable; at other positions a wide range of residues and residue types are tolerated. The number of substitutions allowed at each position roughly correlates with the solvent accessibility of the wild-type side chain.


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