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Science 17 June 1988:
Vol. 240. no. 4859, pp. 1667 - 1669
DOI: 10.1126/science.2454509
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Articles

Science, Vol 240, Issue 4859, 1667-1669
Copyright © 1988 by American Association for the Advancement of Science

articles

cAMP evokes long-term facilitation in Aplysia sensory neurons that requires new protein synthesis

S Schacher, VF Castellucci, and ER Kandel

Center for Neurobiology and Behavior, Howard Hughes Medical Institute, College of Physicians and Surgeons of Columbia University, New York State Psychiatric Institute, NY 10032.

Behavioral sensitization leads to both short- and long-term enhancement of synaptic transmission between the sensory and motor neurons of the gill-withdrawal reflex in Aplysia. Serotonin (5-HT), a transmitter important for short-term sensitization, can evoke long-term enhancement of synaptic strength detected 1 day later. Because 5-HT mediates short-term facilitation through adenosine 3',5'-monophosphate (cAMP)-dependent protein phosphorylation, the role of cAMP in the long-term modulation of this identified synapse was examined. Like 5-HT, cAMP can also evoke long-term facilitation lasting 24 hours. Unlike the short-term change, the long-lasting change is blocked by anisomycin, a reversible inhibitor of protein synthesis, and therefore must involve the synthesis of gene products not required for the short-term change.


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