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Science 10 June 1988:
Vol. 240. no. 4858, pp. 1538 - 1541
DOI: 10.1126/science.2836954
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Articles

Science, Vol 240, Issue 4858, 1538-1541
Copyright © 1988 by American Association for the Advancement of Science

articles

Mitochondrial transformation in yeast by bombardment with microprojectiles

SA Johnston, PQ Anziano, K Shark, JC Sanford, and RA Butow

Department of Botany, Duke University, Durham, NC 27706.

The genetic transformation of mitochondria and chloroplasts has been an intractable problem. The newly developed "biolistic" (biological ballistic) process was used to deliver DNA into yeast cells to stably transform their mitochondria. A nonreverting strain, which is respiratory deficient because of a deletion in the mitochondrial oxi3 gene, was bombarded with tungsten microprojectiles coated with DNA bearing sequences that could correct the oxi3 deletion. Respiratory-competent transformants were obtained in which the introduced oxi3 DNA is integrated at the homologous site in the mitochondrial genome. Organelle genomes can now be manipulated by molecular genetic techniques in the same way as nuclear genomes.


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Mitochondrial transformation in yeast by bombardment with microprojectiles.
S. Johnston, P. Anziano, K Shark, J. Sanford, and R. Butow (1988)
Science 240, 1538-1541
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