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Science 6 May 1988:
Vol. 240. no. 4853, pp. 777 - 779
DOI: 10.1126/science.3363359
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Articles

Science, Vol 240, Issue 4853, 777-779
Copyright © 1988 by American Association for the Advancement of Science

articles

Direct observation of the femtosecond excited-state cis-trans isomerization in bacteriorhodopsin

RA Mathies, CH Brito Cruz, WT Pollard, and CV Shank

Department of Chemistry, University of California, Berkeley 94720.

Femtosecond optical measurement techniques have been used to study the primary photoprocesses in the light-driven transmembrane proton pump bacteriorhodopsin. Light-adapted bacteriorhodopsin was excited with a 60-femtosecond pump pulse at 618 nanometers, and the transient absorption spectra from 560 to 710 nanometers were recorded from -50 to 1000 femtoseconds by means of 6-femtosecond probe pulses. By 60 femtoseconds, a broad transient hole appeared in the absorption spectrum whose amplitude remained constant for about 200 femtoseconds. Stimulated emission in the 660- to 710-nanometer region and excited-state absorption in the 560- to 580-nanometer region appeared promptly and then shifted and decayed from 0 to approximately 150 femtoseconds. These spectral features provide a direct observation of the 13-trans to 13-cis torsional isomerization of the retinal chromophore on the excited-state potential surface. Absorption due to the primary ground-state photoproduct J appears with a time constant of approximately 500 femtoseconds.


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