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Science 22 April 1988:
Vol. 240. no. 4851, pp. 506 - 509
DOI: 10.1126/science.2451860
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Articles

Science, Vol 240, Issue 4851, 506-509
Copyright © 1988 by American Association for the Advancement of Science

articles

Stimulation of RNA and protein synthesis by intracellular insulin

DS Miller

Laboratory of Cellular and Molecular Pharmacology, National Institute of Environmental Health Sciences, Research Triangle Park, NC 27709.

Like insulin-sensitive somatic cells, stage IV oocytes from Xenopus laevis increase their synthesis of RNA, protein, and glycogen in response to extracellular insulin. Synthesis of RNA and protein are also increased when oocytes are maintained under paraffin oil and insulin is microinjected into the cytoplasm. The effects of external and intracellular insulin are additive, suggesting separate mechanisms of action. Experiments with nuclei isolated under oil show that RNA synthesis can be stimulated by applying insulin to the nucleus directly. Thus, the nucleus appears to be one intracellular site of hormone action.


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Human insulin-degrading enzyme shares structural and functional homologies with E. coli protease III.
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