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Science 18 March 1988:
Vol. 239. no. 4846, pp. 1412 - 1416
DOI: 10.1126/science.2450400
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Articles

Science, Vol 239, Issue 4846, 1412-1416
Copyright © 1988 by American Association for the Advancement of Science

articles

RNA as an RNA polymerase: net elongation of an RNA primer catalyzed by the Tetrahymena ribozyme

MD Been and TR Cech

Department of Chemistry and Biochemistry, University of Colorado, Boulder 80309.

A catalytic RNA (ribozyme) derived from an intervening sequence (IVS) RNA of Tetrahymena thermophila will catalyze an RNA polymerization reaction in which pentacytidylic acid (C5) is extended by the successive addition of mononucleotides derived from a guanylyl-(3',5')-nucleotide (GpN). Cytidines or uridines are added to C5 to generate chain lengths of 10 to 11 nucleotides, with longer products being generated at greatly reduced efficiency. The reaction is analogous to that catalyzed by a replicase with C5 acting as the primer, GpNs as the nucleoside triphosphates, and a sequence in the ribozyme providing a template. The demonstration that an RNA enzyme can catalyze net elongation of an RNA primer supports theories of prebiotic RNA self-replication.


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