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Science 18 March 1988:
Vol. 239. no. 4846, pp. 1400 - 1405
DOI: 10.1126/science.2831625

Articles

Science, Vol 239, Issue 4846, 1400-1405
Copyright © 1988 by American Association for the Advancement of Science


articles

Activation of cell-specific expression of rat growth hormone and prolactin genes by a common transcription factor

C Nelson, VR Albert, HP Elsholtz, LI Lu, and MG Rosenfeld

Eukaryotic Regulatory Biology Program, University of California, San Diego, School of Medicine 92093.

In the anterior pituitary gland, there are five phenotypically distinct cell types, including cells that produce either prolactin (lactotrophs) or growth hormone (somatotrophs). Multiple, related cis-active elements that exhibit synergistic interactions appear to be the critical determinants of the transcriptional activation of the rat prolactin and growth hormone genes. A common positive tissue-specific transcription factor, referred to as Pit-1, appears to bind to all the cell-specific elements in each gene and to be required for the activation of both the prolactin and growth hormone genes. The data suggest that, in the course of development, a single tissue-specific factor activates sets of genes that ultimately exhibit restricted cell-specific expression and define cellular phenotype.


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The POU domain: versatility in transcriptional regulation by a flexible two-in-one DNA-binding domain..
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A Dopamine-responsive Domain in the N-terminal Sequence of Pit-1.
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Pit-1 Exhibits a Unique Promoter Spacing Requirement for Activation and Synergism.
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Genes & Dev. 7, 913-932
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A Mutation in the POU-Homeodomain of Pit-1 Responsible for Combined Pituitary Hormone Deficiency.
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Science 257, 1115-1118
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Mutation of the POU-Specific Domain of Pit-1 and Hypopituitarism Without Pituitary Hypoplasia.
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Elk-1, C/EBPalpha , and Pit-1 Confer an Insulin-responsive Phenotype on Prolactin Promoter Expression in Chinese Hamster Ovary Cells and Define the Factors Required for Insulin-increased Transcription.
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